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    products/primary-antibodies/vsv-g-tag-antibody-epr12997-ab183497.pdf

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Tags & Cell Markers Epitope Tags Other Tags VSV-G Tag
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RecombinantRabMAb

Recombinant Anti-VSV-G tag antibody [EPR12997] (ab183497)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (2) Submit a question

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Western blot - Anti-VSV-G tag antibody [EPR12997] (ab183497)
  • Anti-VSV-G tag antibody [EPR12997] (ab183497)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12997] to VSV-G tag
  • Suitable for: WB
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-VSV-G tag antibody [EPR12997]
    See all VSV-G tag primary antibodies
  • Description

    Rabbit monoclonal [EPR12997] to VSV-G tag
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Multi-tag protein lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12997
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • VSV-G Tag

Associated products

  • Alternative Versions

    • Anti-VSV-G tag antibody [EPR12997] - BSA and Azide free (ab250656)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000.
Notes
WB
1/1000 - 1/10000.

Target

  • Relevance

    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names

    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Images

  • Western blot - Anti-VSV-G tag antibody [EPR12997] (ab183497)
    Western blot - Anti-VSV-G tag antibody [EPR12997] (ab183497)
    Anti-VSV-G tag antibody [EPR12997] (ab183497) at 1/1000000 dilution + Multi-tag protein lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  • Anti-VSV-G tag antibody [EPR12997] (ab183497)
    Anti-VSV-G tag antibody [EPR12997] (ab183497)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab183497? Please let us know so that we can cite the reference in this datasheet.

ab183497 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Flow Cytometry abreview for Anti-VSV-G tag antibody [EPR12997]

Good
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (HEK23T)
Permeabilization
No
Gating Strategy
remove debris, separate agregates
Specification
HEK23T
Preparation
Cell harvesting/tissue preparation method: Re-suspension by Trypsine deattatchment
Sample buffer: PBS
Fixation
None
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dimitrie Ivancic

Verified customer

Submitted Sep 28 2021

Western blot abreview for Anti-VSV-G tag antibody [EPR12997]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human immunodeficiency virus Cell lysate - whole cell (viral lysate)
Gel Running Conditions
Reduced Denaturing (4-12 Bis Tris)
Loading amount
5 µg
Specification
viral lysate
Blocking step
Licor intercept TBS as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 29 2020

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