Recombinant Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
![Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-122482-anti-wapl-antibody-epr34072-western-blot.jpg "Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3407(2)] to WAPL/FOE
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-WAPL/FOE antibody [EPR3407(2)]
See all WAPL/FOE primary antibodies -
Description
Rabbit monoclonal [EPR3407(2)] to WAPL/FOE -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, and Jurkat cell lysates. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3407(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/50000 - 1/200000. Predicted molecular weight: 133 kDa.
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
|
WB
1/50000 - 1/200000. Predicted molecular weight: 133 kDa. |
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ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt or IHC-P.
Target
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Function
Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. -
Tissue specificity
Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart. -
Sequence similarities
Contains 1 WAPL domain. -
Cellular localization
Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase. - Information by UniProt
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Database links
- Entrez Gene: 23063 Human
- Omim: 610754 Human
- SwissProt: Q7Z5K2 Human
- Unigene: 203099 Human
- Unigene: 714876 Human
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Alternative names
- FOE antibody
- Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
- Friend of EBNA2 protein antibody
see all
Images
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Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 133 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-122481-anti-wapl-antibody-epr34072-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.
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![Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-122480-anti-wapl-antibody-epr34072-western-blot.jpg)
Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/10000 dilution
Lane 1 : Human U2OS cells treated with siRNA control.
Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE
Lysates/proteins at 90 µg per lane.
Secondary
Lane 1 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Lanes 2-3 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa (possible cross reactivity), 130 kDa (possible cross reactivity), 145 kDa (possible cross reactivity)
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![Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.com/ps/products/109/ab109537/Images/ab109537-4-benefits-of-recombinant-antibodies.png)
Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109537 has been referenced in 2 publications.
- Pinzaru AM et al. Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore. Genes Dev 34:1619-1636 (2020). PubMed: 33122293
- Kanke M et al. Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO J 35:2686-2698 (2016). PubMed: 27872142