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RecombinantRabMAb

Recombinant Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)

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Western blot - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
  • Western blot - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
  • Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
  • Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11350] to WDR5 - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-WDR5 antibody [EPR11350] - BSA and Azide free
    See all WDR5 primary antibodies

  • Description

    Rabbit monoclonal [EPR11350] to WDR5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt (Intra)more details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and Saos-2 cell lysates; Human breast and Human thyroid gland tissues; HeLa cells. RAW264.7 and Mouse spleen, rat spleen lysates.

  • General notes

    ab250036 is the carrier-free version of ab178410.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.

  • Sequence similarities

    Belongs to the WD repeat WDR5/wds family.
    Contains 7 WD repeats.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2410008O07Rik antibody
    • AA408785 antibody
    • AA960360 antibody
    • BIG 3 antibody
    • Big antibody
    • BIG3 antibody
    • BMP2 induced 3 kb gene protein antibody
    • BMP2 induced gene, 3-KB antibody
    • BMP2-induced 3-kb gene protein antibody
    • MGC114572 antibody
    • OTTHUMP00000162494 antibody
    • SWD 3 antibody
    • SWD3 antibody
    • SWD3 Set1c WD40 repeat protein homolog antibody
    • WD repeat containing protein 5 antibody
    • WD repeat domain 5 antibody
    • WD repeat protein 5 antibody
    • WD repeat-containing protein 5 antibody
    • WD repeat-containing protein BIG-3 antibody
    • WDR 5 antibody
    • Wdr5 antibody
    • WDR5_HUMAN antibody
    see all

Images

  • Western blot - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    Western blot - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 36 kDa

  • Western blot - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    Western blot - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 36 kDa
    Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab178410, the same antibody clone in a different buffer formulation.

  • Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)

    This data was developed using ab178410, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometrical analysis of permeabilized HeLa cells labeling WDR5 with ab178410 at 1/10 (red) compared to a nonspecific negative control antibody (green). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    This data was developed using ab178410, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling WDR5 with ab178410 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    This data was developed using ab178410, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human breast tissue labeling WDR5 with ab178410 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)
    Anti-WDR5 antibody [EPR11350] - BSA and Azide free (ab250036)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab250036? Please let us know so that we can cite the reference in this datasheet.

ab250036 has not yet been referenced specifically in any publications.

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