Recombinant Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
![Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)](https://www.abcam.com/ps/products/178/ab178410/Images/ab178410-451685-anti-wdr5-antibody-epr11350-c-terminal-western-blot-raw2647.jpg "Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11350] to WDR5 - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-WDR5 antibody [EPR11350] - C-terminal
See all WDR5 primary antibodies -
Description
Rabbit monoclonal [EPR11350] to WDR5 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human WDR5 aa 300 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: P61964 -
Positive control
- HeLa and Saos-2 cell lysates; Human breast and Human thyroid gland tissues; HeLa cells. RAW264.7 and Mouse spleen, rat spleen lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11350 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. -
Sequence similarities
Belongs to the WD repeat WDR5/wds family.
Contains 7 WD repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11091 Human
- Entrez Gene: 140858 Mouse
- Entrez Gene: 362093 Rat
- Omim: 609012 Human
- SwissProt: P61964 Human
- SwissProt: P61965 Mouse
- SwissProt: Q498M4 Rat
- Unigene: 397638 Human
see all -
Alternative names
- 2410008O07Rik antibody
- AA408785 antibody
- AA960360 antibody
see all
Images
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Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 36 kDa -
![Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)](https://www.abcam.com/ps/products/178/ab178410/Images/ab178410-206281-anti-wdr5-antibody-epr11350-c-terminal-western-blot.jpg)
Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Saos-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands. -
![Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)](https://www.abcam.com/ps/products/178/ab178410/Images/ab178410-206284-anti-wdr5-antibody-epr11350-c-terminal-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)Intracellular flow cytometrical analysis of permeabilized HeLa cells labeling WDR5 with ab178410 at 1/10 (red) compared to a nonspecific negative control antibody (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)](https://www.abcam.com/ps/products/178/ab178410/Images/ab178410-206283-anti-wdr5-antibody-epr11350-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling WDR5 with ab178410 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)](https://www.abcam.com/ps/products/178/ab178410/Images/ab178410-206282-anti-wdr5-antibody-epr11350-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)Immunohistochemical analysis of paraffin embedded Human breast tissue labeling WDR5 with ab178410 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)](https://www.abcam.com/ps/products/178/ab178410/Images/ab178410-5-benefits-of-recombinant-antibodies.png)
Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab178410 has been referenced in 9 publications.
- Zhang C et al. Furin extracellularly cleaves secreted PTENα/β to generate C-terminal fragment with a tumor-suppressive role. Cell Death Dis 13:532 (2022). PubMed: 35668069
- Yuan J et al. Inhibition of Wdr5 Attenuates Ang-II-Induced Fibroblast-to-Myofibroblast Transition in Cardiac Fibrosis by Regulating Mdm2/P53/P21 Pathway. Biomolecules 12:N/A (2022). PubMed: 36358925
- Zhang Y et al. LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940. J Hepatocell Carcinoma 8:333-348 (2021). PubMed: 33977095
- Zhao J et al. Structural insights into the recognition of histone H3Q5 serotonylation by WDR5. Sci Adv 7:N/A (2021). PubMed: 34144982
- Chen T et al. WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways. Mol Ther 29:2134-2150 (2021). PubMed: 33601056
- Feng Z et al. Mixed Lineage Leukemia 1 Promoted Neuron Apoptosis in Ischemic Penumbra via Regulating ASK-1/TNF-a Complex. Front Neuroanat 14:36 (2020). PubMed: 32792914
- Cui Z et al. Effect of high WDR5 expression on the hepatocellular carcinoma prognosis. Oncol Lett 15:7864-7870 (2018). PubMed: 29731905
- Hoshii T et al. A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Cell 172:1007-1021.e17 (2018). PubMed: 29474905
- Barbieri I et al. Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control. Nature 552:126-131 (2017). PubMed: 29186125