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RecombinantRabMAb

Recombinant Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

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Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
  • Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
  • Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
  • Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
  • Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10709(2)] to WDR77 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free
    See all WDR77 primary antibodies

  • Description

    Rabbit monoclonal [EPR10709(2)] to WDR77 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251023 is the carrier-free version of ab190361.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

  • Tissue specificity

    Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.

  • Sequence similarities

    Contains 5 WD repeats.

  • Developmental stage

    Expressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.

  • Cellular localization

    Nucleus. Cytoplasm. Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2610312E17Rik antibody
    • Androgen receptor cofactor p44 antibody
    • C79984 antibody
    • HKMT1069 antibody
    • MEP 50 antibody
    • MEP-50 antibody
    • MEP50 antibody
    • MEP50_HUMAN antibody
    • Methylosome protein 50 antibody
    • MGC2722 antibody
    • Nbla10071 antibody
    • p44 antibody
    • p44/Mep50 antibody
    • RGD1310479 antibody
    • RP11 552M11.3 antibody
    • WD repeat containing protein 77 antibody
    • WD repeat domain 77 antibody
    • WD repeat-containing protein 77 antibody
    • WDR77 antibody
    see all

Images

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/10000 dilution

    Lane 1 : LnCaP cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    This data was developed using ab190361, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilution + Saos-2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    This data was developed using ab190361, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    This data was developed using ab190361, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    This data was developed using ab190361, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling WDR77 with ab190361 at 1/50 and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    This data was developed using ab190361, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of A673 cells fixed in 4% paraformaldehyde labeling WDR77 with ab190361 at 1/50 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) 1/200. Counterstained with DAPI. Negative controls also shown.
  • Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

    This data was developed using ab190361, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling WDR77 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG used as isotype control. 

     

     

  • Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    This data was developed using ab190361, the same antibody clone in a different buffer formulation.Immunoprecipitation of HeLa cells labeling WDR77 with ab190361 at 1/50 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000 (+) or PBS (-)
  • Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab251023? Please let us know so that we can cite the reference in this datasheet.

ab251023 has not yet been referenced specifically in any publications.

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