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RecombinantRabMAb

Recombinant Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

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Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
  • Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
  • Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
  • Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
  • Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10709(2)] to WDR77 - C-terminal
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-WDR77 antibody [EPR10709(2)] - C-terminal
    See all WDR77 primary antibodies

  • Description

    Rabbit monoclonal [EPR10709(2)] to WDR77 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • LnCaP, 293T, HeLa, Saos-2, C6, Raw 264.7, PC-12 and NIH-3T3 cell lysates, Human adenocarcinoma of colon tissue. A673 and 293T cells,

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/40.
ICC/IF
1/50.
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/40.
ICC/IF
1/50.
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

  • Tissue specificity

    Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.

  • Sequence similarities

    Contains 5 WD repeats.

  • Developmental stage

    Expressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.

  • Cellular localization

    Nucleus. Cytoplasm. Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2610312E17Rik antibody
    • Androgen receptor cofactor p44 antibody
    • C79984 antibody
    • HKMT1069 antibody
    • MEP 50 antibody
    • MEP-50 antibody
    • MEP50 antibody
    • MEP50_HUMAN antibody
    • Methylosome protein 50 antibody
    • MGC2722 antibody
    • Nbla10071 antibody
    • p44 antibody
    • p44/Mep50 antibody
    • RGD1310479 antibody
    • RP11 552M11.3 antibody
    • WD repeat containing protein 77 antibody
    • WD repeat domain 77 antibody
    • WD repeat-containing protein 77 antibody
    • WDR77 antibody
    see all

Images

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/10000 dilution

    Lane 1 : LnCaP cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilution + Saos-2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling WDR77 with ab190361 at 1/50 and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

    Immunofluorescence analysis of A673 cells fixed in 4% paraformaldehyde labeling WDR77 with ab190361 at 1/50 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) 1/200. Counterstained with DAPI. Negative controls also shown.

  • Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling WDR77 at 1/70 dilution andGoat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG used as isotype control. 

     

     

  • Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

    Immunoprecipitation of HeLa cells labeling WDR77 with ab190361 at 1/50 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000  (+)  or PBS (-)

  • Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)
    Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361)

References (0)

Publishing research using ab190361? Please let us know so that we can cite the reference in this datasheet.

ab190361 has not yet been referenced specifically in any publications.

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