Recombinant Anti-Wee1 antibody [EPR20899] (ab233540)
![Western blot - Anti-Wee1 antibody [EPR20899] (ab233540)](https://www.abcam.com/ps/products/233/ab233540/Images/ab233540-338748-anti-wee1-antibody-epr20899-western-blot.jpg "Western blot - Anti-Wee1 antibody [EPR20899] (ab233540)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20899] to Wee1
- Suitable for: WB
- Knockout validated
- Reacts with: Schizosaccharomyces pombe
Related conjugates and formulations
Overview
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Product name
Anti-Wee1 antibody [EPR20899]
See all Wee1 primary antibodies -
Description
Rabbit monoclonal [EPR20899] to Wee1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Schizosaccharomyces pombe -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Fission yeast (Schizosaccharomyces pombe) whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20899 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 96 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 96 kDa). |
Target
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Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated during M and G1 phases. Also autophosphorylated.
Ubiquitinated and degraded at the onset of G2/M phase. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- DKFZp686I18166 antibody
- EC 2.7.10.2 antibody
- FLJ16446 antibody
see all
Images
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Western blot - Anti-Wee1 antibody [EPR20899] (ab233540)All lanes : Anti-Wee1 antibody [EPR20899] (ab233540) at 1/1000 dilution
Lane 1 : Wild-type Schizosaccharomyces pombe whole cell lysate
Lane 2 : Wee1 knockout Schizosaccharomyces pombe whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 96 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsBlocking and dilution buffer: 5% NFDM/TBST.
ab233540 was shown to specifically react with Wee1 in wild-type fission yeast cells as signal was lost in Wee1 knockout cells. Wild-type and Wee1 knockout samples were subjected to SDS-PAGE. ab233540 and ab210797 (Rabbit anti-Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Wee1 knockout and WT fission yeast whole cell lysates were kindly provided by Dr Quanwen Jin.
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![Anti-Wee1 antibody [EPR20899] (ab233540)](https://www.abcam.com/ps/products/233/ab233540/Images/ab233540-2-benefits-of-recombinant-antibodies.png)
Anti-Wee1 antibody [EPR20899] (ab233540)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab233540 has been referenced in 2 publications.
- Xiao R et al. Inhibiting the IRE1α Axis of the Unfolded Protein Response Enhances the Antitumor Effect of AZD1775 in TP53 Mutant Ovarian Cancer. Adv Sci (Weinh) 9:e2105469 (2022). PubMed: 35619328
- Jin H et al. A distinct class of plant and animal viral proteins that disrupt mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1. Sci Adv 6:eaba3418 (2020). PubMed: 32426509