Recombinant Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)
![Western blot - Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)](https://www.abcam.com/ps/products/245/ab245987/Images/ab245987-338943-anti-wee1-antibody-epr20899-western-blot.jpg "Western blot - Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20899] to Wee1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Schizosaccharomyces pombe
Related conjugates and formulations
Overview
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Product name
Anti-Wee1 antibody [EPR20899] - BSA and Azide free
See all Wee1 primary antibodies -
Description
Rabbit monoclonal [EPR20899] to Wee1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Schizosaccharomyces pombe -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Fission yeast (Schizosaccharomyces pombe) whole cell lysate.
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General notes
ab245987 is the carrier-free version of ab233540.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20899 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 96 kDa).
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 96 kDa). |
Target
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Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated during M and G1 phases. Also autophosphorylated.
Ubiquitinated and degraded at the onset of G2/M phase. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- DKFZp686I18166 antibody
- EC 2.7.10.2 antibody
- FLJ16446 antibody
see all
Images
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Western blot - Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)All lanes : Anti-Wee1 antibody [EPR20899] (ab233540) at 1/1000 dilution
Lane 1 : Wild-type Schizosaccharomyces pombe whole cell lysate
Lane 2 : Wee1 knockout Schizosaccharomyces pombe whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 96 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsBlocking and dilution buffer: 5% NFDM/TBST.
ab233540 was shown to specifically react with Wee1 in wild-type fission yeast cells as signal was lost in Wee1 knockout cells. Wild-type and Wee1 knockout samples were subjected to SDS-PAGE. ab233540 and ab210797 (Rabbit anti-Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Wee1 knockout and WT fission yeast whole cell lysates were kindly provided by Dr Quanwen Jin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233540).
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![Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)](https://www.abcam.com/ps/products/245/ab245987/Images/ab245987-2-benefits-of-recombinant-antibodies.png)
Anti-Wee1 antibody [EPR20899] - BSA and Azide free (ab245987)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab245987 has not yet been referenced specifically in any publications.
