Recombinant Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)
![Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)](https://www.abcam.com/ps/products/124/ab124673/Images/ab124673-387832-anti-werners-syndrome-helicase-wrn-antibody-epr6392-western-blot-hap1-wt-hap1-wrn-knockout-molt4-k562.jpg "Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6392] to Werner's syndrome helicase WRN
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Werner's syndrome helicase WRN antibody [EPR6392]
See all Werner's syndrome helicase WRN primary antibodies -
Description
Rabbit monoclonal [EPR6392] to Werner's syndrome helicase WRN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Werner's syndrome helicase WRN aa 1400-1500 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HAP1, MOLT 4, K562 and A431 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6392 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa). |
Target
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Function
Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. -
Involvement in disease
Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500]. -
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 3'-5' exonuclease domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7486 Human
- Omim: 604611 Human
- SwissProt: Q14191 Human
- Unigene: 632050 Human
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Alternative names
- DKFZp686C2056 antibody
- DNA helicase antibody
- DNA helicase, RecQ like type 3 antibody
see all
Images
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Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : WRN knockout HAP1 cell lysate
Lane 3 : MOLT-4 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 162 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab124673 was shown to react with Werner's syndrome helicase WRN in wild-type HAP1 cells in western blot. Loss of signal was observed when WRN knockout sample was used. Wild-type and WRN knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124673 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)](https://www.abcam.com/ps/products/124/ab124673/Images/ab124673-259130-anti-werners-syndrome-helicase-wrn-antibody-epr6392-western-blot.jpg)
Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Werner's syndrome helicase WRN knockout HAP1 cell lysate (20 µg)
Lane 3: MOLT4 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab181602, observed at 124 kDa.
ab124673 was shown to recognize Werner's syndrome helicase WRN when Werner's syndrome helicase WRN knockout samples were used, along with additional cross-reactive bands. Wild-type and Werner's syndrome helicase WRN knockout samples were subjected to SDS-PAGE. ab124673 and ab181602 (loading control to GAPDH) were both diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)](https://www.abcam.com/ps/products/124/ab124673/Images/ab124673-138408-anti-werners-syndrome-helicase-wrn-antibody-epr6392-western-blot.jpg)
Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution
Lane 1 : MOLT4 cell lysates
Lane 2 : K562 cell lysates
Lane 3 : A431 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 162 kDa -
![Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)](https://www.abcam.com/ps/products/124/ab124673/Images/ab124673-4-benefits-of-recombinant-antibodies.png)
Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124673 has been referenced in 2 publications.
- Romero-Zamora D & Hayashi MT A non-catalytic N-terminus domain of WRN prevents mitotic telomere deprotection. Sci Rep 13:645 (2023). PubMed: 36635307
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371