Recombinant Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666)
![Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666)](https://www.abcam.com/ps/products/187/ab187666/Images/ab187666-220680-anti-wisp3-antibody-epr14769-c-terminal-western-blot.jpg "Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14769] to WISP3 - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-WISP3 antibody [EPR14769] - C-terminal
See all WISP3 primary antibodies -
Description
Rabbit monoclonal [EPR14769] to WISP3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Caco-2, C6 and HepG2 whole cell lysate (ab7900); Mouse heart, mouse brain and rat brain lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14769 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa). |
Target
-
Function
Appears to be required for normal postnatal skeletal growth and cartilage homeostasis. -
Tissue specificity
Predominant expression in adult kidney and testis and fetal kidney. Weaker expression found in placenta, ovary, prostate and small intestine. Also expressed in skeletally-derived cells such as synoviocytes and articular cartilage chondrocytes. -
Involvement in disease
Defects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC) [MIM:208230]. PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 8838 Human
- Entrez Gene: 327743 Mouse
- Entrez Gene: 499461 Rat
- Omim: 603400 Human
- SwissProt: O95389 Human
- SwissProt: D3Z5L9 Mouse
- Unigene: 558428 Human
-
Alternative names
- CCN 6 antibody
- CCN family member 6 antibody
- CCN6 antibody
see all
Images
-
Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666)All lanes : Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666) at 1/1000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666)](https://www.abcam.com/ps/products/187/ab187666/Images/ab187666-220678-anti-wisp3-antibody-epr14769-c-terminal-western-blot.jpg)
Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666)All lanes : Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
![Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666)](https://www.abcam.com/ps/products/187/ab187666/Images/ab187666-3-benefits-of-recombinant-antibodies.png)
Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666)
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab187666 has been referenced in 1 publication.
- McMullen ER et al. CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast. Breast Cancer Res Treat 172:577-586 (2018). PubMed: 30220054