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    products/primary-antibodies/zbtb40-antibody-c-terminal-ab190185.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-ZBTB40 antibody - C-terminal (ab190185)

  • Datasheet
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Western blot - Anti-ZBTB40 antibody - C-terminal (ab190185)

    Key features and details

    • Rabbit polyclonal to ZBTB40 - C-terminal
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-ZBTB40 antibody - C-terminal
      See all ZBTB40 primary antibodies
    • Description

      Rabbit polyclonal to ZBTB40 - C-terminal
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      Recombinant fragment corresponding to Human ZBTB40 aa 950 to the C-terminus (C terminal).
      Database link: Q9NUA8

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • U2OS cell lysate, Mouse embrio fibroblasts cell lysate and INS1 cell lysate
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab190185 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/2000. Predicted molecular weight: 138 kDa.
    Notes
    WB
    1/2000. Predicted molecular weight: 138 kDa.

    Target

    • Function

      May be involved in transcriptional regulation.
    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 1 BTB (POZ) domain.
      Contains 12 C2H2-type zinc fingers.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9NUA8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9923 Human
      • Entrez Gene: 230848 Mouse
      • Entrez Gene: 362635 Rat
      • Omim: 612106 Human
      • SwissProt: Q9NUA8 Human
      • Unigene: 418966 Human
      • Alternative names

        • BC059177 antibody
        • C230087D24 antibody
        • Gm571 antibody
        • KIAA0478 antibody
        • MGC133098 antibody
        • MGC62412 antibody
        • mKIAA0478 antibody
        • RGD1309866 antibody
        • RP23 95O23.1 antibody
        • ZBT40_HUMAN antibody
        • ZBTB40 antibody
        • Zinc finger and BTB domain containing 40 antibody
        • Zinc finger and BTB domain containing protein 40 antibody
        • Zinc finger and BTB domain-containing protein 40 antibody
        • ZNF923 antibody
        see all

      Images

      • Western blot - Anti-ZBTB40 antibody - C-terminal (ab190185)
        Western blot - Anti-ZBTB40 antibody - C-terminal (ab190185)
        All lanes : Anti-ZBTB40 antibody - C-terminal (ab190185) at 1/2000 dilution

        Lane 1 : U2OS cell lysate
        Lane 2 : U2OS cells transfected with ZBTB40 siRNA
        Lane 3 : Mouse embrio fibroblasts (MEFs) cell lysate
        Lane 4 : INS1 cell lysate

        Lysates/proteins at 40 µg per lane.

        Predicted band size: 138 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (1)

      Publishing research using ab190185? Please let us know so that we can cite the reference in this datasheet.

      ab190185 has been referenced in 1 publication.

      • Doolittle ML  et al. Genetic analysis of osteoblast activity identifies Zbtb40 as a regulator of osteoblast activity and bone mass. PLoS Genet 16:e1008805 (2020). WB ; Mouse . PubMed: 32497039

      Customer reviews and Q&As

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