Anti-ZBTB46 antibody (ab222830)
Key features and details
- Rabbit polyclonal to ZBTB46
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZBTB46 antibody
See all ZBTB46 primary antibodies -
Description
Rabbit polyclonal to ZBTB46 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human ZBTB46 aa 150-400.
Database link: Q86UZ6 -
Positive control
- WB: HEK-293T and SH-SY5Y cell lysates; Mouse heart, liver and kidney tissue lysates. IHC-P: Human brain and rectum tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/20 - 1/200.
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| WB |
1/1000 - 1/5000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
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| Notes |
|---|
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IHC-P
1/20 - 1/200. |
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WB
1/1000 - 1/5000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
Target
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Relevance
ZBTB46 may be involved in transcriptional regulation. It contains one BTB (POZ) domain and two C2H2 type zinc fingers. -
Cellular localization
Nucleus -
Database links
- Entrez Gene: 140685 Human
- Entrez Gene: 72147 Mouse
- SwissProt: Q86UZ6 Human
- SwissProt: Q8BID6 Mouse
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Alternative names
- BTB/POZ domain containing protein 4 antibody
- BTBD4 antibody
- RINZF antibody
see all
Images
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Western blot - Anti-ZBTB46 antibody (ab222830)All lanes : Anti-ZBTB46 antibody (ab222830) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB46 antibody (ab222830)Paraffin-embedded human brain tissue stained for ZBTB46 using ab222830 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB46 antibody (ab222830)Paraffin-embedded human rectum tissue stained for ZBTB46 using ab222830 at 1/100 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab222830 has not yet been referenced specifically in any publications.