Recombinant Anti-ZPI antibody [EPR23683-118] (ab273627)
![Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-395033-anti-zpi-antibody-epr23683118-western-blot-human-serum20-ul.jpg "Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23683-118] to ZPI
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ZPI antibody [EPR23683-118]
See all ZPI primary antibodies -
Description
Rabbit monoclonal [EPR23683-118] to ZPI -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat plasma. Human liver cancer tissue lysate. Human serum. IHC-P: Human liver, pancreas and placenta tissue; Rat liver tissue. IP: Human plasma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23683-118 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 50 kDa).
|
| Notes |
|---|
|
IP
1/30. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 50 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 51156 Human
- Entrez Gene: 217847 Mouse
- Entrez Gene: 100909524 Rat
- Entrez Gene: 171154 Rat
- Omim: 605271 Human
- SwissProt: Q9UK55 Human
- SwissProt: Q8R121 Mouse
- SwissProt: Q62975 Rat
see all -
Alternative names
- Protein Z dependent protease inhibitor antibody
- Protein Z dependent protease inhibitor precursor antibody
- Protein Z-dependent protease inhibitor antibody
see all
Images
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Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627)All lanes : Anti-ZPI antibody [EPR23683-118] (ab273627) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lysates/proteins at 20 µl per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:10460162, 9689066).
Exposure time: 3 minutes.
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![Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-395034-anti-zpi-antibody-epr23683118-western-blot-human-liver-cancer-tissue-lysate20-ug.jpg)
Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627)All lanes : Anti-ZPI antibody [EPR23683-118] (ab273627) at 1/1000 dilution
Lane 1 : Human liver cancer tissue lysate
Lane 2 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 10460162, 9689066).
Negative control: Human brain (PMID: 10460162).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 37 seconds.
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![Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-395035-anti-zpi-antibody-epr23683118-western-blot-mouse-plasma20-ul.jpg)
Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627)All lanes : Anti-ZPI antibody [EPR23683-118] (ab273627) at 1/1000 dilution
Lane 1 : Mouse plasma
Lane 2 : Rat serum
Lane 3 : Rat plasma
Lysates/proteins at 20 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:10460162, 9689066).
Exposure times: Lane 1: 26 seconds; Lane 2-3: 5.5 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-1-anti-zpi-antibody-epr23683118-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human liver (PMID: 20024489). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-2-anti-zpi-antibody-epr23683118-immunohistochemistry-human-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on pancreas islets (PMID: 16728581). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-3-anti-zpi-antibody-epr23683118-immunohistochemistry-human-placenta.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma in human placenta. The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-4-anti-zpi-antibody-epr23683118-immunohistochemistry-rat-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat liver (PMID: 20024489). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-5-anti-zpi-antibody-epr23683118-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (ab273627)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: No staining on human cererbum (PMID: 20024489).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunoprecipitation - Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-6-anti-zpi-antibody-epr23683118-immunoprecipitation-human-plasma-lysate-10-ug.jpg)
Immunoprecipitation - Anti-ZPI antibody [EPR23683-118] (ab273627)ZPI was immunoprecipitated from 0.35 mg human plasma with ab273627 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273627 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human plasma lysate 10µg
Lane 2: ab273627 IP in human plasma
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273627 in human plasma
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile observed is consistent with what has been described in the literature (PMID:10460162).
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![Anti-ZPI antibody [EPR23683-118] (ab273627)](https://www.abcam.com/ps/products/273/ab273627/Images/ab273627-11-benefits-of-recombinant-antibodies.png)
Anti-ZPI antibody [EPR23683-118] (ab273627)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab273627 has not yet been referenced specifically in any publications.