ChIC/CUT&RUN pAG-MNase (ab285373)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
Description
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Product name
ChIC/CUT&RUN pAG-MNase -
Biological activity
pAG-MNase enzyme for performing ChIC/CUT&RUN experiments. Biological activity determined by using pAG-MNase in the ChIC/CUT&RUN experiment on 1 X105 HeLa cells with ab188408 Anti-CTCF antibody followed by sequencing.
pAG-MNase is supplied in 10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50% glycerol. Use 2 µl pAG-MNase per 50 µl ChIC/CUT&RUN reaction (25X dilution).
Number of reactions:
100 µl vial (50 reactions)
500 µl vial (250 reactions)
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Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Additional sequence information
Nuclease
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Specifications
Our Abpromise guarantee covers the use of ab285373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Form
Liquid -
Additional notes
ChIC was first described by the Laemmli group in 2004 (1). The ChIC method involves cleavage of double-stranded DNA (1). The antibody directed pAG-MNAse fusion protein (ab285373) results in 100 – 200 bp resolution mapping of DNA-bound proteins (1, 2). ChIC/CUT&RUN-seq allows chromatin immunoprecipitation to be performed on fewer than 100 cells for histone modifications and ~1000 cells for transcription factors compared to ChIP-seq which generally needs >106 cells (1, 3). Another advantage of ChIC/CUT&RUN-seq over ChIP-seq is an ~10-fold lower sequencing depth (1, 3). Abcam provides an increasing number of ChIC/CUT&RUN-seq validated antibodies to meet the needs of this emerging technique.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - ChIC/CUT&RUN pAG-MNase (ab285373)
Figure 1: Protein gel of pAG-MNAse
pAG-MNase ab285373 (16 µg per lane) was analysed by SDS-PAGE and stained using Coomassie Blue. The molecular weights are outlined alongside the target protein.
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Functional Studies - ChIC/CUT&RUN pAG-MNase (ab285373)Figure 2: Size distribution of cleaved chromatin
ChIC/CUT&RUN was performed with ab285373 pAG-MNase (1:25 dilution) and Anti-CTCF antibody (ab188408). Library preparation was performed and the size of excised DNA fragments was analysed by an Agilent 4200 TapeStation. Enriched cleaved DNA mononucleosomes are observed at ~300 bp.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285373 has not yet been referenced specifically in any publications.