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  1. Link

    products/proteins-peptides/cx3cr1-peptide-ab8125.pdf

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Microbiology Interspecies Interaction Host Virus Interaction
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CX3CR1 peptide (ab8125)

  • Datasheet
Reviews (1)Q&A (1)References (1)

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Western blot - CX3CR1 peptide (ab8125)

    Key features and details

    • Suitable for: Blocking, WB

    You may also be interested in

    Peptide
    CCR2 peptide (ab178768)
    Protein
    Recombinant human CX3CL1 protein (ab9820)

    View more associated products

    Description

    • Product name

      CX3CR1 peptide
      See all CX3CR1 proteins and peptides
    • Purity

      95 % HPLC.

    • Animal free

      No
    • Nature

      Synthetic
      • Sequence

        DQFPESVTENFEYDDLAEAC

    Specifications

    Our Abpromise guarantee covers the use of ab8125 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituent: 0.1% BSA

    General Info

    • Alternative names

      • Beta chemokine receptor-like 1
      • C X3 C CKR 1
      • C-X3-C CKR-1
      • CCRL1
      • Chemokine C X3 C motif receptor 1
      • CMK BRL 1
      • CMK-BRL-1
      • CMK-BRL1
      • CMKBLR1
      • CMKDR1
      • CX3C chemokine receptor 1
      • CX3C CKR1
      • CX3C1_HUMAN
      • CX3CR1
      • Fractalkine receptor
      • G-protein coupled receptor 13
      • GPR13
      • GPRV28
      • V28
      see all
    • Function

      Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
    • Tissue specificity

      Expressed in lymphoid and neural tissues.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P49238 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - CX3CR1 peptide (ab8125)
      Western blot - CX3CR1 peptide (ab8125)
      All lanes : CX3CR1 antibody

      Lane 1 : Human spleen lysate
      Lane 2 : Human spleen lysate with CX3CR1 peptide (ab8125)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab8125? Please let us know so that we can cite the reference in this datasheet.

    ab8125 has been referenced in 1 publication.

    • Shen F  et al. Novel Small-Molecule CX3CR1 Antagonist Impairs Metastatic Seeding and Colonization of Breast Cancer Cells. Mol Cancer Res 14:518-27 (2016). PubMed: 27001765

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    Other (Immunohistochemistry peptide block) Abreview for CX3CR1 peptide (Human)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Other - Do not use

    Review text: Used as a control for blocking the activity of anti-CX3CR1 (ab8021) in immunohistochemistry frozen sections. Peptide was incubated with an equal volume of antibody at 37*C for 30 mins before applying to sections diluted 1:400 in PBS.
    Sample: Mouse Tissue sections

    Primary antibody (in addition to 'CX3CR1 peptide (Human)')
    Primary antibody: Abcam primary antibody: Anti-CX3CR1 antibody (ab8021)
    Dilution: 1/400

    Secondary antibody
    Name: Non-Abcam antibody was used: Alexa Fluor® 568 goat anti-rabbit IgG (H+L) *2 mg/
    Host species: Goat
    Clonality: Polyclonal
    Conjugation: Alexa Fluor® 568
    Dilution: 1/800
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 30 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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