Human BCL9 peptide (ab37304)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking, WB
Description
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Product name
Human BCL9 peptide
See all BCL9 proteins and peptides -
Purity
> 90 % HPLC. -
Animal free
No -
Nature
Synthetic -
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Species
Human
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Associated products
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Corresponding Antibody
Specifications
Our Abpromise guarantee covers the use of ab37304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Blocking - Blocking peptide for Anti-BCL9 antibody (ab37305)
Western blot
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Form
Liquid -
Additional notes
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
General Info
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Alternative names
- B-cell CLL/lymphoma 9
- BCL 9
- Legless homolog
- LGS
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Relevance
BCL9 is involved in signal transduction through the Wnt pathway. It promotes beta-catenin's transcriptional activity. BCL9 is associated with B-cell acute lymphoblastic leukemia. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies (referenced from swissprot and entrez gene). -
Cellular localization
Nuclear
Images
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Blocking - Human BCL9 peptide (ab37304)Image from Takada K et al., Sci Transl Med. 2012 Aug 22;4(148):148ra117. doi: 10.1126/scitranslmed.3003808.; Fig S1.; Sci Transl Med 22 August 2012: Vol. 4, Issue 148, p. 148ra117.BCL9 staining in Human colorectal cancer tissue, blocked with ab37304.
Left panel: ab37305 - BCL9 antibody alone. Right panel: BCL9 antibody + ab37304 - BCL9 peptide.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab37304 has been referenced in 2 publications.
- Takada K et al. Targeted disruption of the BCL9/ß-catenin complex inhibits oncogenic Wnt signaling. Sci Transl Med 4:148ra117 (2012). Blocking ; Human . PubMed: 22914623
- Mani M et al. BCL9 promotes tumor progression by conferring enhanced proliferative, metastatic, and angiogenic properties to cancer cells. Cancer Res 69:7577-86 (2009). WB . PubMed: 19738061
