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    products/proteins-peptides/human-bcl9-peptide-ab37304.pdf

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Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Proto-oncogenes
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Human BCL9 peptide (ab37304)

  • Datasheet
Submit a review Submit a question References (2)

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Blocking - Human BCL9 peptide (ab37304)

    Key features and details

    • Purity: > 90% HPLC
    • Suitable for: Blocking, WB

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    Description

    • Product name

      Human BCL9 peptide
      See all BCL9 proteins and peptides
    • Purity

      > 90 % HPLC.

    • Animal free

      No
    • Nature

      Synthetic
      • Species

        Human

    Associated products

    • Corresponding Antibody

      • Anti-BCL9 antibody (ab37305)

    Specifications

    Our Abpromise guarantee covers the use of ab37304 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-BCL9 antibody (ab37305)

      Western blot

    • Form

      Liquid
    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    General Info

    • Alternative names

      • B-cell CLL/lymphoma 9
      • BCL 9
      • Legless homolog
      • LGS
    • Relevance

      BCL9 is involved in signal transduction through the Wnt pathway. It promotes beta-catenin's transcriptional activity. BCL9 is associated with B-cell acute lymphoblastic leukemia. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies (referenced from swissprot and entrez gene).
    • Cellular localization

      Nuclear

    Images

    • Blocking - Human BCL9 peptide (ab37304)
      Blocking - Human BCL9 peptide (ab37304)Image from Takada K et al., Sci Transl Med. 2012 Aug 22;4(148):148ra117. doi: 10.1126/scitranslmed.3003808.; Fig S1.; Sci Transl Med 22 August 2012: Vol. 4, Issue 148, p. 148ra117.
      BCL9 staining in Human colorectal cancer tissue, blocked with ab37304.

      Left panel: ab37305 - BCL9 antibody alone. Right panel: BCL9 antibody + ab37304 - BCL9 peptide.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab37304? Please let us know so that we can cite the reference in this datasheet.

    ab37304 has been referenced in 2 publications.

    • Takada K  et al. Targeted disruption of the BCL9/ß-catenin complex inhibits oncogenic Wnt signaling. Sci Transl Med 4:148ra117 (2012). Blocking ; Human . PubMed: 22914623
    • Mani M  et al. BCL9 promotes tumor progression by conferring enhanced proliferative, metastatic, and angiogenic properties to cancer cells. Cancer Res 69:7577-86 (2009). WB . PubMed: 19738061

    Customer reviews and Q&As

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