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  1. Link

    products/proteins-peptides/human-beta-actin-peptide-ab13772.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Human beta Actin peptide (ab13772)

  • Datasheet
Reviews (1) Submit a question References (5)

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Western blot - Human beta Actin peptide (ab13772)

    Key features and details

    • Purity: > 90% HPLC
    • Suitable for: Blocking

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    Description

    • Product name

      Human beta Actin peptide
      See all beta Actin proteins and peptides
    • Purity

      > 90 % HPLC.

    • Accession

      P60709
    • Animal free

      No
    • Nature

      Synthetic
      • Species

        Human

    Associated products

    • Corresponding Antibody

      • Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
      • Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
      • Anti-beta Actin antibody (ab8227)
      • Anti-beta Actin antibody - Loading Control (ab8229)

    Specifications

    Our Abpromise guarantee covers the use of ab13772 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224), Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226), Anti-beta Actin antibody (ab8227), Anti-beta Actin antibody - Loading Control (ab8229)

    • Form

      Lyophilized
    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C.

      Information available upon request.

    General Info

    • Alternative names

      • A26C1A
      • A26C1B
      • ACTB
      • ACTB_HUMAN
      • Actin beta
      • Actin cytoplasmic 1
      • Actin, cytoplasmic 1, N-terminally processed
      • Actx
      • b actin
      • b-actin
      • Beta cytoskeletal actin
      • Beta-actin
      • BRWS1
      • E430023M04Rik
      • Melanoma X actin
      • MGC128179
      • PS1TP5 binding protein 1
      • PS1TP5BP1
      see all
    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease

      Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
    • Sequence similarities

      Belongs to the actin family.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Target information above from: UniProt accession P60709 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Human beta Actin peptide (ab13772)
      Western blot - Human beta Actin peptide (ab13772)
      All lanes : Anti-beta Actin antibody (ab8227) at 1/1000 dilution

      Lane 1 : HeLa nuclear lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : HEK 293 cell lysate
      Lane 6 : HeLa nuclear lysate with Human beta Actin peptide (ab13772) at 1 µg/ml
      Lane 7 : HeLa whole cell lysate with Human beta Actin peptide (ab13772) at 1 µg/ml
      Lane 8 : A431 cell lysate with Human beta Actin peptide (ab13772) at 1 µg/ml
      Lane 9 : Jurkate cell lysate with Human beta Actin peptide (ab13772) at 1 µg/ml
      Lane 10 : HEK 293 cell lysate with Human beta Actin peptide (ab13772) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

      Exposure time: 5 seconds

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (5)

    Publishing research using ab13772? Please let us know so that we can cite the reference in this datasheet.

    ab13772 has been referenced in 5 publications.

    • Pasquettaz R  et al. Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma. J Comp Neurol 529:553-575 (2021). PubMed: 32515035
    • Carley E  et al. The LINC complex transmits integrin-dependent tension to the nuclear lamina and represses epidermal differentiation. Elife 10:N/A (2021). PubMed: 33779546
    • Sun D  et al. Acanthopanax senticosus polysaccharide suppressing proliferation and metastasis of the human non-small cell lung cancer NCI-H520 cells is associated with Wnt/ß-catenin signaling. Neoplasma 66:555-563 (2019). PubMed: 30943746
    • Zeng S  et al. Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/ß-catenin pathway. Mol Med Rep 18:5572-5578 (2018). PubMed: 30365080
    • Dinter J  et al. Different antigen-processing activities in dendritic cells, macrophages, and monocytes lead to uneven production of HIV epitopes and affect CTL recognition. J Immunol 193:4322-34 (2014). PubMed: 25230751

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Blocking peptide for human beta actin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Other - Do not use
    Lane 1 : Fresh Antibody
    Lane 2 : Fresh Antibody (Incubation 45 minutes at room temperature)
    Lane 3 : Only Second Antibody
    Lane 4 : Antibody : Antigen = 1:5 (Incubation 45 minutes at room temperature)
    Lane 5 : Antibody : Antigen = 1: 0.05 (Incubation 45 minutes at room temperature)
    Lane 6 : Antibody : Antigen = 1: 0.0005 (Incubation 45 minutes at room temperature)
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 12 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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