Human beta Actin peptide (ab13772)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
Description
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Product name
Human beta Actin peptide
See all beta Actin proteins and peptides -
Purity
> 90 % HPLC. -
Accession
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Animal free
No -
Nature
Synthetic -
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Species
Human
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Associated products
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Corresponding Antibody
Specifications
Our Abpromise guarantee covers the use of ab13772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
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Form
Lyophilized -
Additional notes
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
Information available upon request.
General Info
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Alternative names
- A26C1A
- A26C1B
- ACTB
see all -
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
Images
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Western blot - Human beta Actin peptide (ab13772)All lanes : Anti-beta Actin antibody (ab8227) at 1/1000 dilution
Lane 1 : HeLa nuclear lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK 293 cell lysate
Lane 6 : HeLa nuclear lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lane 7 : HeLa whole cell lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lane 8 : A431 cell lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lane 9 : Jurkate cell lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lane 10 : HEK 293 cell lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Exposure time: 5 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab13772 has been referenced in 5 publications.
- Pasquettaz R et al. Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma. J Comp Neurol 529:553-575 (2021). PubMed: 32515035
- Carley E et al. The LINC complex transmits integrin-dependent tension to the nuclear lamina and represses epidermal differentiation. Elife 10:N/A (2021). PubMed: 33779546
- Sun D et al. Acanthopanax senticosus polysaccharide suppressing proliferation and metastasis of the human non-small cell lung cancer NCI-H520 cells is associated with Wnt/ß-catenin signaling. Neoplasma 66:555-563 (2019). PubMed: 30943746
- Zeng S et al. Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/ß-catenin pathway. Mol Med Rep 18:5572-5578 (2018). PubMed: 30365080
- Dinter J et al. Different antigen-processing activities in dendritic cells, macrophages, and monocytes lead to uneven production of HIV epitopes and affect CTL recognition. J Immunol 193:4322-34 (2014). PubMed: 25230751
