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  1. Link

    products/proteins-peptides/human-eaat2-peptide-ab41752.pdf

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Neuroscience Neurotransmitter Amino Acids Glutamate
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Human EAAT2 peptide (ab41752)

  • Datasheet
Reviews (1) Submit a question References (1)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Purity: > 90% n/a
  • Suitable for: Blocking

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Primary
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Anti-EAAT1 antibody [EPR12686] (ab181036)
Primary
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Anti-EAAT2 antibody (ab41621)
Peptide
Human EAAT1 peptide (ab42682)

View more associated products

Description

  • Product name

    Human EAAT2 peptide
  • Purity

    > 90 % n/a.

  • Accession

    P43004
  • Animal free

    No
  • Nature

    Synthetic
    • Species

      Human

Associated products

  • Corresponding Antibody

    • Anti-EAAT2 antibody (ab41621)

Specifications

Our Abpromise guarantee covers the use of ab41752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-EAAT2 antibody (ab41621)

  • Form

    Lyophilized
  • Additional notes

    Reconstitution instructions:

     -  First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.

    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.

    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.

    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.

    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names

    • EAA2_HUMAN
    • EAAT2
    • Excitatory amino acid transporter 2
    • Excitotoxic amino acid transporter 2
    • Glial high affinity glutamate transporter
    • GLT 1
    • GLT1
    • Glutamate aspartate transporter II
    • Glutamate transporter 1
    • Glutamate/aspartate transporter II
    • Slc1a2
    • Sodium dependent glutamate aspartate transporter 2
    • Sodium-dependent glutamate/aspartate transporter 2
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2
    • Solute carrier family 1 glial high affinity glutamate transporter member 2
    • Solute carrier family 1 member 2
    see all
  • Function

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Sequence similarities

    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P43004 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab41752? Please let us know so that we can cite the reference in this datasheet.

ab41752 has been referenced in 1 publication.

  • Potapenko ES  et al. Altered astrocyte glutamate transporter regulation of hypothalamic neurosecretory neurons in heart failure rats. Am J Physiol Regul Integr Comp Physiol 303:R291-300 (2012). PubMed: 22696576

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Blocking - Blocking peptide for Anti-EAAT2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Other - Do not use
Predicted band size would be 62 kDa.
however observed band size for cow liver is less then 62 kDa.
Loading amount of each lane : 60ug of cow liver
1 lane: Fresh Primary antibody (ab41621)
2 lane: Fresh Primary antibody with 45 min incubation at RT
3 lane: only Secondary antibody (without primary antibody)
4 lane: Primary antibody : Peptide = 1 : 0.01with 45 min incubation at RT
5 lane: Primary antibody : Peptide = 1 : 0.001with 45 min incubation at RT
6 lane: Primary antibody : Peptide = 1 : 0.0001with 45 min incubation at RT
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Kwangwon Son

Verified customer

Submitted Nov 16 2017

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