Human Hepcidin-25 peptide (ab31875)
Key features and details
- Purity: > 70% HPLC
- Suitable for: Blocking, ELISA
Product nameHuman Hepcidin-25 peptide
Purity> 70 % HPLC.
70 - 90% by HPLC
Our Abpromise guarantee covers the use of ab31875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Hepcidin-25 antibody (ab30760)
Storage buffer: 10% 0.5M acetic acid, 90% TK buffer. pH 3.6
Solutions of ab31875, diluted 10 times, in TBST (80%) and PBS (10%) have a pH of 5.1
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
ReconstitutionReconstitute in 10% 0.5M acetic acid, 90% TK buffer. If the peptide doesn’t dissolve try an organic solvent like DMSO, then dilute using water or buffer. Gentle warming and sonication can effectively aid peptide solubilisation
FunctionLiver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
Tissue specificityHighest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
Involvement in diseaseHemochromatosis 2B
Sequence similaritiesBelongs to the hepcidin family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
ab31875 has been referenced in 4 publications.
- Zhou X et al. Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes. Aging (Albany NY) 13:26063-26094 (2021). PubMed: 34954691
- Xiao L et al. Macrophage iron retention aggravates atherosclerosis: Evidence for the role of autocrine formation of hepcidin in plaque macrophages. Biochim Biophys Acta Mol Cell Biol Lipids 1865:158531 (2020). PubMed: 31666189
- Xiong XY et al. Toll-Like Receptor 4/MyD88-Mediated Signaling of Hepcidin Expression Causing Brain Iron Accumulation, Oxidative Injury, and Cognitive Impairment After Intracerebral Hemorrhage. Circulation 134:1025-1038 (2016). PubMed: 27576776
- McCarthy RC & Kosman DJ Glial cell ceruloplasmin and hepcidin differentially regulate iron efflux from brain microvascular endothelial cells. PLoS One 9:e89003 (2014). PubMed: 24533165