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Native cow Folate Binding Protein/FBP (ab82272)

Submit a review Q&A (1)References (1)

Key features and details

  • Expression system: Native
  • Purity: > 95% HPLC
  • Active: Yes

Description

  • Product name

    Native cow Folate Binding Protein/FBP
    See all Folate Binding Protein/FBP proteins and peptides

  • Biological activity

    Activity: 18.6ug/ml (Folate binding)

  • Purity

    > 95 % HPLC.

  • Expression system

    Native

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Species

      Cow

    • Additional sequence information

      Source = bovine milk

Specifications

Our Abpromise guarantee covers the use of ab82272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form

    Liquid

  • Additional notes

    Source: bovine milk. Protein content: 2.3mg/ml (indirectly using the measured activity in ug/ml and the binding capacity of 8ug folic acid/mg Folate binding protein.) .

    Previously labelled as Folate Binding Protein

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 1.968% Sodium phosphate, 0.174% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • adult
    • Adult folate binding protein
    • Adult folate-binding protein
    • FBP
    • Folate Binding Protein
    • Folate receptor
    • Folate receptor 1
    • Folate Receptor 1 Adult
    • Folate Receptor 1 Precursor
    • Folate receptor adult
    • Folate receptor alpha
    • FOLR
    • FOLR1
    • FOLR1_HUMAN
    • FR alpha
    • FR-alpha
    • FRalpha
    • KB cells FBP
    • MOV18
    • Ovarian cancer associated antigen
    • Ovarian tumor associated antigen
    • Ovarian tumor associated antigen MOv18
    • Ovarian tumor-associated antigen MOv18
    see all

  • Function

    Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.

  • Tissue specificity

    Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.

  • Involvement in disease

    Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.

  • Sequence similarities

    Belongs to the folate receptor family.

  • Post-translational
    modifications

    Eight disulfide bonds are present.
    The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.

  • Cellular localization

    Cell membrane. Secreted.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab82272? Please let us know so that we can cite the reference in this datasheet.

ab82272 has been referenced in 1 publication.

  • Kim HS  et al. Fibronectin type III and intracellular domains of Toll-like receptor 4 interactor with leucine-rich repeats (Tril) are required for developmental signaling. Mol Biol Cell 29:523-531 (2018). PubMed: 29298840

Customer reviews and Q&As

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Question

Customer contacted us as the datasheet states: Protein content: 2.3mg/ml (indirectly using the measured activity in ug/ml and the binding capacity of 8ug folic acid/mg Folate binding protein.). Could we give details of how that was performed?

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Answer

Thank you for contacting us. I have had the opportunity to discuss this with the lab.

The protein concentration of this product is not measured directly. The protein concentration is calculated from the folate binding activity (ug folic acid bound per mL; determined by a folate binding assay) divided by the binding capacity of folate binding protein (assume 8ug folic acid per mg folate binding protein).



As an example for a recent lot :

Measured Activity of 14.8ug/mL (14.8ug folic acid bound per mL)

Assume binding capacity of 8ug folic acid per mg folate binding protein

14.8ug/ml activity divided by 8ug/mg binding capacity = 1.85mg/mL Folate Binding Protein



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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