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  1. Link

    products/proteins-peptides/native-human-collagen-i-protein-ab7533.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Native Human Collagen I protein (ab7533)

  • Datasheet
  • SDS
Reviews (2)Q&A (6)References (4)

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Western blot - Native Human Collagen I protein (ab7533)

    Key features and details

    • Expression system: Native
    • Purity: > 95% n/a
    • Suitable for: WB, IP, ELISA

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    Description

    • Product name

      Native Human Collagen I protein
      See all Collagen I proteins and peptides
    • Purity

      > 95 % n/a.
      This product has been prepared from human placenta by pepsin digestion and is chromatographically and immunologically pure.
    • Expression system

      Native
    • Accession

      P02452
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Native
      • Species

        Human
      • Predicted molecular weight

        139 kDa

    Associated products

    • Related Products

      • Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)

    Specifications

    Our Abpromise guarantee covers the use of ab7533 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      Immunoprecipitation

      ELISA

    • Form

      Liquid
    • Additional notes

      This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Preservative: 0.01% Sodium azide
      Constituent: 0.003% Acetic acid

    General Info

    • Alternative names

      • Alpha 1 type I collagen
      • Alpha 2 type I collagen
      • alpha 2 type I procollagen
      • alpha 2(I) procollagen
      • alpha 2(I)-collagen
      • Alpha-1 type I collagen
      • alpha1(I) procollagen
      • CO1A1_HUMAN
      • COL1A1
      • COL1A2
      • collagen 1
      • collagen alpha 1 chain type I
      • Collagen alpha-1(I) chain
      • collagen alpha-1(I) chain preproprotein
      • Collagen I alpha 1 polypeptide
      • Collagen I alpha 2 polypeptide
      • collagen of skin, tendon and bone, alpha-1 chain
      • collagen of skin, tendon and bone, alpha-2 chain
      • Collagen type I alpha 1
      • Collagen type I alpha 2
      • EDSC
      • OI1
      • OI2
      • OI3
      • OI4
      • pro-alpha-1 collagen type 1
      • type I proalpha 1
      • Type I procollagen
      • type I procollagen alpha 1 chain
      see all
    • Function

      Type I collagen is a member of group I collagen (fibrillar forming collagen).
    • Tissue specificity

      Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
    • Involvement in disease

      Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
      Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
      Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
      Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
      Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
    • Sequence similarities

      Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 VWFC domain.
    • Post-translational
      modifications

      Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
      O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P02452 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Native Human Collagen I protein (ab7533)
      Western blot - Native Human Collagen I protein (ab7533)
      Native Human Collagen I protein (ab7533) at 1/1000 dilution + Human Collagen at 0.05 µg

      Secondary
      DyLight™ 649 Rabbit Secondary Antibody at 1/20000 dilution

      Observed band size: 130 kDa why is the actual band size different from the predicted?

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab7533? Please let us know so that we can cite the reference in this datasheet.

    ab7533 has been referenced in 4 publications.

    • Spiers RM  et al. Donor age significantly influences the Raman spectroscopic biomolecular fingerprint of human pancreatic extracellular matrix proteins following collagenase-based digestion. Acta Biomater 99:269-283 (2019). PubMed: 31525537
    • Long KR  et al. Extracellular Matrix Components HAPLN1, Lumican, and Collagen I Cause Hyaluronic Acid-Dependent Folding of the Developing Human Neocortex. Neuron 99:702-719.e6 (2018). PubMed: 30078576
    • Kehlet SN  et al. A fragment of SPARC reflecting increased collagen affinity shows pathological relevance in lung cancer - implications of a new collagen chaperone function of SPARC. Cancer Biol Ther 19:904-912 (2018). PubMed: 30067436
    • Wan F  et al. Calpastatin overexpression impairs postinfarct scar healing in mice by compromising reparative immune cell recruitment and activation. Am J Physiol Heart Circ Physiol 309:H1883-93 (2015). PubMed: 26453333

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Sandwich ELISA for the detection of human collagen I

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sandwich ELISA for the detection of human collagen I, using Human anti-collagen I antibody ab6308 and anti-collagen I antibody biotin ab2482.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 06 2017

    Human collagen type 1

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    SDS-PAGE
    Loading amount: 250 ng
    Gel: Reduced denaturing (4-12% Bis-Tris gel (Novex))
    Lane 1 standards
    Lane 2 ab7533
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 22 2015

    Question

    I am using the Collagen I standard (Collagen I protein (ab7533) ) and noticed that anti-collagen I, anti-collagen III, and anti-collagen IV antibodies all detect it in a dot blot.  Does this standard contain other types of collagen besides Collagen I?  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 16 2011

    Answer

    Our collagen I should not contain significant amounts of other types of collagen, however, the subunit strands of the different collagen types are similar and may share epitopes in common.  If the antibodies used are not type specific this can cause reduced specificity.  Our collagens are purified in a manner to select protein which has preserved 3D structure of type specific epitopes.

    Read More

    Abcam Scientific Support

    Answered on Dec 16 2011

    Question

    I hve a question concerning ab7533 " human collagen I isolated from placenta". How is it isolated from the placenta? with acid? with proteolytic enzymes? with something else?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 02 2007

    Answer

    Regarding ab7533 (collagen I protein), this product has been prepared from human placenta by pepsin digestion. Please don't hesitate to contact us again if you have further questions.

    Read More

    Abcam Scientific Support

    Answered on Jan 02 2007

    Question

    what is the cross reactivity of ab7533 and ab7535 with collagen type I?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 15 2005

    Answer

    These products have been prepared from human placenta and are chromatographically and immunologically pure. They are free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Reaction with our anti-Collagen antibodies I, II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA) apart from the expect cross reaction with the collagen type i.e ab7535 cross reacts with the anti-collagen III antibody, and ab7533 cross reacts with the anti-collagen I antibody.

    Read More

    Abcam Scientific Support

    Answered on Jul 15 2005

    Question

    I am using ab7533 for western analysis on rat fibroblasts (dilution of 1:100) and I see a predominant band at ~83 kDa with a weak band at ~134 kDa. Do you know what the band is at 83 kDa?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 08 2003

    Answer

    Our data shows there are two major bands at 129kDa and 138kDa under non-reducing condition but we are not aware of a band appearing at 83kDa. We suggest non-denaturing, non-dessociating gel for Collagens.

    Read More

    Abcam Scientific Support

    Answered on Jul 09 2003

    Question

    I want to know about ab7533 collagen type I human protein. Ab292, collagen type I antibody, can react with this protein in ELISA or western blot?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 24 2002

    Answer

    Ab292 will react with ab7533 in western blotting and ELISA.

    Read More

    Abcam Scientific Support

    Answered on Jun 24 2002

    Question

    When you run this product, collagen I, on an SDS gel under reducing conditions, what are the molecular weights?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 13 2002

    Answer

    The answer to this question depends on several varibles such as source, method of preparation, and how they were stored and handled, etc. One gel shows Collagen I has two major bands, one at 138kDa for its a1(I) chains and one at 129kDa for its a2(I)chain. There are some aggregated collagen bands in high molecular weight region.

    Read More

    Danielle Miller

    Abcam Scientific Support

    Answered on Mar 19 2002

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