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Native human hCG beta protein (ab126653)

Submit a review Q&A (8)References (3)

Key features and details

  • Expression system: Native
  • Purity: >= 98% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE

Description

  • Product name

    Native human hCG beta protein
    See all hCG beta proteins and peptides

  • Purity

    >= 98 % SDS-PAGE.
    Chromatographic techniques

  • Expression system

    Native

  • Accession

    P01233

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Species

      Human

    • Predicted molecular weight

      22 kDa

    • Amino acids

      21 to 165

Associated products

Specifications

Our Abpromise guarantee covers the use of ab126653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized

  • Additional notes

    All Human source materials have tested negative for HIV, HCV, and HAV antibodies, HBsAg, and HIV (PCR). No test guarantees a product to be non-infectious. Therefore, all material derived from Human fluids or tissues should be considered as potentially infectious.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Constituent: 0.4% Ammonium bicarbonate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute using neutral pH buffer of choice. Avoid extreme high or low pH.

General Info

  • Alternative names

    • CG beta
    • CG-beta
    • CgB
    • CGB3
    • CGB3_HUMAN
    • CGB5
    • CGB7
    • CGB7_HUMAN
    • CGB8
    • CGHB_HUMAN
    • Choriogonadotropin subunit beta
    • Choriogonadotropin subunit beta 3
    • Choriogonadotropin subunit beta 7
    • Chorionic gonadotropin beta polypeptide
    • Chorionic gonadotropin chain beta
    see all

  • Relevance

    Function: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Tissue specificity: Placenta. Similarity: Belongs to the glycoprotein hormones subunit beta family. Developmental stage: Made by the first trimester placenta.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab126653? Please let us know so that we can cite the reference in this datasheet.

ab126653 has been referenced in 3 publications.

  • Hires M  et al. Glycan signatures for the identification of cisplatin-resistant testicular cancer cell lines: Specific glycoprofiling of human chorionic gonadotropin (hCG). Cancer Med 11:968-982 (2022). PubMed: 35044085
  • Sarkar M  et al. Progesterone limits the tumor-promoting effects of the beta-subunit of human chorionic gonadotropin via non-nuclear receptors. iScience 25:104527 (2022). PubMed: 35754725
  • Sengodan SK  et al. ß-hCG-induced mutant BRCA1 ignites drug resistance in susceptible breast tissue. Carcinogenesis 40:1415-1426 (2019). PubMed: 30963174

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Question

Could you send the reference for specific binding between ab400 and ab126653 in general notes of antibody datasheet for me?
I am worried that it cannot bind together.

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Answer

The antibody ab400 was tested for specific binding to hCG, but not the hCG we supply as ab126653. There is no published journal reference for the testing of the antibody ab400, and I am fairly certain there is no commercial source of the hCG protein that was used for the testing. However, I am confident that ab400 will bind to ab126653.

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Question

I would like to know whether ab400 can bind ab126653, yes or not ?

In the case of ab126653 cannot bind to ab400, please recommend other BhCG protein that can definitely bind to ab400 antibody.

The application used is SPR/ELISA.

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Answer

We have not tested the two products together, ab400 antibody and ab126653 protein, but we assume that ab126653 is almost identical, if not exactly identical, to the hCG beta that was used to determine the binding constants listed in the general notes section of the antibody datasheet.

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Question

What is the pI value (Isoelectric point) of ab 126653?

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Answer

The pI value (isoelectric point) range of ab126653 hCG beta protein is 8.0-9.0 with a calculated pI of 8.32.

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Question

Vielen Danke für Ihre schnelle Antwort. Anbei das Bild meines letzten SDS-PAGE Gels. Auf dieses Gel habe ich reines hCG beta geladen, sowie eine Probe einer deglycosylierungs-Reaktion mit PNGase F. Bei der reinen hCG beta Probe sind zwei Banden mit ca. 28 kDa, bzw. 19 kDa Molekulargewicht erkennbar. Durch die Deglycosylierung verschiebt sich das MW der Bande mit dem höheren MW von ca. 28 kDa auf ca. 22 kDa, das MW der zweiten Bande verändert sich nicht. Ich vermute daher, dass es sich bei der zweiten Bande um eine weiter deglycosylierte Variante handelt.

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Answer

Vielen Dank für Ihre Anfrage und Kooperation.


Leider hat mein Nachhacken ergeben, dass die Charge, die Sie erhalten haben, mit hCG alpha kontaminiert ist. Wir werden daher eine neue Charge erhalten, die ich natürlich, wenn Sie dies den möchten, an Sie weiter leiten werde.

Bitte lassen Sie mich wissen, ob dies für Sie in Frage kommt oder ob wir uns eine Alternative überlegen müssen.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Question

Ich habe nochmals eine Frage hinsichtlich des hCG-beta proteins. Bei einem SDS-Gel mit reduzierter Probe zeigten sich nämlich 2 Banden mit einem Molekulargewicht von ca. 15 kDa und ca. 20 kDa. Da ich nicht von einer Verunreinigung ausgehe, vermute ich, dass es sich hierbei vielleicht um die glykosylierte und nicht glykosylierte Form von hCG beta handelt. Es würde mich sehr freuen, wenn Sie mir hierbei behilflich sein könnten.

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Answer

Vielen Dank fuer Ihre Email.

Ich habe Ihre Frage an das Labor weiteregeleitet, und diese wuerde sich gerne mal Ihr SDS-Page Gel ansehen.

Koennten Sie mir bitte ein Bild zukommen lassen?

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Question

Has the glycosylation status been checked? Is it possible to get an image of the SDS page?

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Answer

Ich habe endlich eine Antwort von einem der Labore erhalten, die dieses Protein herstellen.
Ich vermute allerdings, sie wird Ihnen nicht gefallen:
"The glycosylation status is not checked for Catalog ab126653 hCG beta protein (Human). An SDS-PAGE image is not available for this product."
Ich warte leider immer noch auf eine Antwort bezüglich des ab96167 hCG beta protein.

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Question

Why is the molecular weight so different?

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Answer

Das unterschiedliche Gewicht erklärt sich daher, dass Molekulargewicht für den ab96167 einfach nur berechnet wurde: die 145 AS der Sequenz haben ein Gewicht von 15531.97 Da.

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Question

Iwould like to know thatthe product:ab96167 can react with the product:ab27242 and ab31206.
another question, How to different betweenthe product:ab126653 and ab96167.

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Answer


ab31206 is an anti-hCG beta conjugated to 40nm gold particles. There is no need to use a secondary antibody such as ab27242.

Yes, the anti-hCG beta (conjugated to gold particles) ab31206 is compatible with both hCG beta proteins ab96167and ab126653.

ab96167 and ab126653 are the same, both are hCG beta protein. The unit sizes are different : ab96167 is 1mg,ab126653 is 100µg.

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