Key features and details
- Expression system: Native
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 25.000 Eu/mg
- Suitable for: ELISA, ICC, SDS-PAGE
Product nameNative Human IgE protein
See all IgE proteins and peptides
Purity> 90 % SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
Endotoxin level< 25.000 Eu/mg
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab152001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesab152001 is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B. Must be handled as potentially infectious as all Human material
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.88% Sodium chloride
- Immunoglobulin heavy constant epsilon
RelevanceIgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
Cellular localizationCell Membrane
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab152001 has been referenced in 1 publication.
- Wagemakers M et al. COX-2 Inhibition Combined with Radiation Reduces Orthotopic Glioma Outgrowth by Targeting the Tumor Vasculature. Transl Oncol 2:1-7 (2009). PubMed: 19252746