Native Human IgE protein (ab152001)
Key features and details
- Expression system: Native
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 25.000 Eu/mg
- Suitable for: ELISA, ICC, SDS-PAGE
Description
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Product name
Native Human IgE protein
See all IgE proteins and peptides -
Purity
> 90 % SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum. -
Endotoxin level
< 25.000 Eu/mg -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human
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Specifications
Our Abpromise guarantee covers the use of ab152001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Immunocytochemistry
SDS-PAGE
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Form
Liquid -
Additional notes
ab152001 is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B. Must be handled as potentially infectious as all Human material -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.88% Sodium chloride
General Info
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Alternative names
- Igh2
- IGHE
- IGHEP1
- Immunoglobulin heavy constant epsilon
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Relevance
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
Cellular localization
Cell Membrane
Images
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SDS-PAGE - Native Human IgE protein (ab152001)
SDS-PAGE analysis of ab152001.
Lane 1: Marker
Lane 2: purified Human IgE (ab152001), MW 185 kDa
Lane 3: Myeloma source IgE, purified from Human serum.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab152001 has been referenced in 1 publication.
- Wagemakers M et al. COX-2 Inhibition Combined with Radiation Reduces Orthotopic Glioma Outgrowth by Targeting the Tumor Vasculature. Transl Oncol 2:1-7 (2009). PubMed: 19252746