For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/proteins-peptides/native-human-ige-protein-azide-free-ab65866.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgE
Share by email

Native Human IgE protein (Azide free) (ab65866)

  • Datasheet
  • SDS
Reviews (2)Q&A (9)References (10)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Sandwich ELISA - Native Human IgE protein (Azide free) (ab65866)
  • SDS-PAGE - Native Human IgE protein (Azide free) (ab65866)

Key features and details

  • Expression system: Native
  • Purity: > 90% SDS-PAGE
  • Endotoxin level: < 25.000 Eu/mg
  • Active: Yes
  • Suitable for: SDS-PAGE, IHC - Wholemount, WB, ELISA, ICC

You may also be interested in

Protein
Product image
Recombinant Human IgG4 Fc Protein, Tag Free (Active) (ab181968)
Primary
Product image
Anti-PMCA1 antibody [EPR12029] (ab190355)
ELISA
Product image
Human IgE ELISA Kit (ab195216)

View more associated products

Description

  • Product name

    Native Human IgE protein (Azide free)
    See all IgE proteins and peptides
  • Biological activity

    1 mg of ab65866 is 380,000± 50,000 IU/m.

  • Purity

    > 90 % SDS-PAGE.
    Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
  • Endotoxin level

    < 25.000 Eu/mg
  • Expression system

    Native
  • Accession

    P0DOX4
    P0DOX7
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Amino Acid Sequence 1

    • Species

      Human
    • Additional sequence information

      Observed band of ~185kDa on SDS-PAGE
    • Amino Acid Sequence 2

    • Species

      Human
  • Description

    Native human IgE protein (Azide free)

Specifications

Our Abpromise guarantee covers the use of ab65866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    IHC - Wholemount

    Western blot

    ELISA

    Immunocytochemistry

  • Form

    Liquid
  • Additional notes

    This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

    This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.

    - Low batch-to-batch variation

    - Higher degree of purity (>90%)

    - A more uniform product (monoclonal and not polyclonal)

    Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 0.00017% PBS, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Igh2
    • IGHE
    • IGHEP1
    • Immunoglobulin heavy constant epsilon
  • Relevance

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization

    Cell Membrane

Images

  • Sandwich ELISA - Native Human IgE protein (Azide free) (ab65866)
    Sandwich ELISA - Native Human IgE protein (Azide free) (ab65866)

    Background-subtracted data values (mean +/- SD) are graphed.

  • SDS-PAGE - Native Human IgE protein (Azide free) (ab65866)
    SDS-PAGE - Native Human IgE protein (Azide free) (ab65866)

    SDS-PAGE analysis of:

    Lane 1: Molecular weight markers.
    Lane 2: ab65866.
    Lane 3: IgE purified from serum from a myeloma patient.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab65866? Please let us know so that we can cite the reference in this datasheet.

ab65866 has been referenced in 10 publications.

  • Lindenburg L  et al. Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins. Nucleic Acids Res 48:e63 (2020). PubMed: 32383757
  • Lafleur MA  et al. MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilities. J Immunotoxicol 17:110-121 (2020). PubMed: 32525431
  • Hotta F  et al. Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear. Int J Mol Med 46:1367-1376 (2020). PubMed: 32945368
  • Vidal M  et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). PubMed: 29397157
  • Wan Z  et al. Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing. PLoS One 13:e0191987 (2018). PubMed: 29389948
  • Khramtsov P  et al. Highly Stable Conjugates of Carbon Nanoparticles with DNA Aptamers. Langmuir N/A:N/A (2018). PubMed: 30089209
  • Cui L  et al. JSI-124 inhibits IgE production in an IgE B cell line. Biochem Biophys Res Commun 483:669-673 (2017). PubMed: 27988336
  • van Beek N  et al. Correlation of Serum Levels of IgE Autoantibodies Against BP180 With Bullous Pemphigoid Disease Activity. JAMA Dermatol N/A:N/A (2016). PubMed: 27829102
  • Baltabekova AZh  et al. SplitCore Technology Allows Efficient Production of Virus-Like Particles Presenting a Receptor-Contacting Epitope of Human IgE. Mol Biotechnol 57:746-55 (2015). PubMed: 25837568
  • Goodman RE  et al. Evaluation of endogenous allergens for the safety evaluation of genetically engineered food crops: review of potential risks, test methods, examples and relevance. J Agric Food Chem 61:8317-32 (2013). SDS-PAGE . PubMed: 23848840

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 11 Abreviews or Q&A

Gold Nanoclusters as fluorescent labels for Human IgE determination

Good
Abreviews
Abreviews
abreview image
Application
Competitive ELISA
We developed a quantitative and sensitive immunoassay to determine Human IgE in human serum using gold nanoclusters (AuNCs) as fluorescent labels. The synthesized AuNCs have a particle size of 2.7 ± 0.1 nm, a maximum fluorescence emission at 710 nm and high stability. The covalent bioconjugation of these AuNCs with the antibody was carried out by the carbodiimide reaction. A molar ratio 1:3 (antibody:AuNCs) was selected.


Human IgE from Abcam (ab65866) was used as standard to prepare the calibration curve in PBS and in human serum matrix (attached figure). Two immunoassay configurations were assayed (competitive and sandwich format), obtaining better detection limit with the competitive format (0.2 ng/mL). For both formats the detection limits were better than those obtained with current commercial ELISA kits (12 ng/mL).

Moreover, the developed fluorescent competitive immunoassay offers other interesting advantages such as fewer reagents and simpler procedures.

The applicability of this new AuNCs immunoprobe was successfully demonstrated for the determination of IgE in human serum, using the competitive configuration.

This research has been published in Biosensors and Bioelectronics,

http://dx.doi.org/10.1016/j.bios.2015.08.011

Authors: María Cruz Alonso, Laura Trapiella-Alfonso, José M Costa Fernández, Rosario Pereiro, Alfredo Sanz-Medel

Title: “Functionalized gold nanoclusters as fluorescent labels for immunoassays: Application to Human serum immunoglobulin E determination”
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

María Cruz

Verified customer

Submitted Sep 29 2015

The hIgE is good

Good
Abreviews
Abreviews
Application
ELISA
The hIgE works in my assay. But the labelled concentration is different from the concentration measured by BCA method. I recommend that measuring the concenration before using it.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 03 2014

Question

What are the masses of the molecular weight markers in the commassie stained gel image for this product?

Read More

Abcam community

Verified customer

Asked on Apr 10 2014

Answer

The masses in the molecular weight marker are as follows, heaviest to lightest in kDa:

1. 195
2. 142
3. 96
4. 71 (Dark band)
5. 48
6. 33
7. 28
8. 22

Read More

Jeremy Kasanov

Abcam Scientific Support

Answered on Apr 10 2014

Question

I am looking for an IgE to use as a standard for a quantification assay for a human IgE biotherapeutic. I saw one from yourselves which said it was good for quantification assays, but was 100ug at 1mg/ml. Do you one like this only with a higher concentration as I think the highest concentration on my standard curve needs to be at 2mg/ml, it is for a hplc assay so it would need to be at least a 1ml quantity.

Read More

Abcam community

Verified customer

Asked on Oct 17 2012

Answer

I am sorry to confirm we are not able to sell this antibody at 1 mg/ml. I can suggest to consider trying concentrating the protein once you receive it.

Please note that as far as we are aware it has not been tested by the application you wish to use.

Read More

Abcam Scientific Support

Answered on Oct 17 2012

Question

In regards to ab65866, are endotoxin levels measured? If so, do you have the levels available?

Read More

Abcam community

Verified customer

Asked on Oct 08 2012

Answer

The lab let me know that they do not measure endotoxin levels in any of their protein solutions and hence they cannot give us a specific value for ab65866.

Read More

Abcam Scientific Support

Answered on Oct 08 2012

Question

Have you assigned a purity grading to ab65866?

Read More

Abcam community

Verified customer

Asked on Feb 02 2012

Answer

I can confirm that ab65866 Human IgE protein has a purity of >95% as determined by SDS PAGE.
I am sorry it has not been given a purity 'grading'.

Read More

Abcam Scientific Support

Answered on Feb 02 2012

Question

Is ab65866 produced from the HE1 clone?

Read More

Abcam community

Verified customer

Asked on Feb 01 2012

Answer

Yes, ab65866 is produced from the HE1 clone.

Read More

Abcam Scientific Support

Answered on Feb 01 2012

Question

How was ab65866 quantified? Was the WHO IgE Serum Standard 75/502 used to standardize?

Read More

Abcam community

Verified customer

Asked on Dec 12 2011

Answer

The antigen is produced in a monoclonal hybridoma, and is not purified from blood or anything else, so the concentration is determined by measuring OD 280. It is then sold based on mg/mL and not in units as the WHO standard product.

Read More

Abcam Scientific Support

Answered on Dec 12 2011

Question

I have a question regarding ab65866. What allergen is specific for this Human IgE protein?

Read More

Abcam community

Verified customer

Asked on Oct 12 2011

Answer

The ab65866 Human IgE protein is an affinity-purified, fully human IgE antibody with kappa light chains. The IgE is produced in vitro from a monoclonal hybridoma. The hybridoma is made by purifying IgE producing B cells from a patient with multiple Myeloma and using this to create a monoclonal hybridoma (cell line producing IgE). I can confirm there is no allergen, as the patient suffers from multiple myeloma (non-specific allergic response).

Read More

Abcam Scientific Support

Answered on Oct 12 2011

Question

Can this protien stimulate mast cells?

Read More

Abcam community

Verified customer

Asked on Sep 30 2011

Answer

Yes, this protein can stimulate mast cells.

Read More

Abcam Scientific Support

Answered on Sep 30 2011

1-10 of 11 Abreviews or Q&A

  •  Previous
  • 1
  • 2
  • Next 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.