Native Human IgG1 protein (ab90283)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, ELISA
Description
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Product name
Native Human IgG1 protein
See all IgG1 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Predicted molecular weight
146 kDa -
Additional sequence information
This product is the whole IgG molecule composed of 2 heavy chains and 2 light chains, joined via disulfide bonds.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab90283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
ELISA
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Form
Liquid -
Additional notes
Protein Determination: Extinction Coeficient (E) 0.1% at 280nm =1.36.
Prepared from myeloma plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3% Sodium phosphate monobasic, monohydrate
General Info
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Alternative names
- Ig gamma 1 chain C region
- IgG heavy chain locus
- IGHG1
see all -
Relevance
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). -
Cellular localization
Secreted. Cell membrane; Single-pass membrane protein
Images
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ELISA - Native Human IgG1 protein (ab90283)
The wells were coated with ab200699 at 5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG1 (ab90283) was then added starting at 100 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab201485 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab90283 has been referenced in 7 publications.
- Lu CH et al. The Fc?RIII Engagement Augments PMA-Stimulated Neutrophil Extracellular Traps (NETs) Formation by Granulocytes Partially via Cross-Talk between Syk-ERK-NF-?B and PKC-ROS Signaling Pathways. Biomedicines 9:N/A (2021). PubMed: 34572313
- Deb C et al. A 1-minute blood test detects decreased immune function and increased clinical risk in COVID-19 patients. Sci Rep 11:23491 (2021). PubMed: 34873223
- Horns F et al. Memory B Cell Activation, Broad Anti-influenza Antibodies, and Bystander Activation Revealed by Single-Cell Transcriptomics. Cell Rep 30:905-913.e6 (2020). PubMed: 31968262
- Henson D et al. Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report. Arterioscler Thromb Vasc Biol 39:1884-1892 (2019). PubMed: 31315438
- Vidal M et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). PubMed: 29397157
- Sinkovits G et al. Concentration and Subclass Distribution of Anti-ADAMTS13 IgG Autoantibodies in Different Stages of Acquired Idiopathic Thrombotic Thrombocytopenic Purpura. Front Immunol 9:1646 (2018). PubMed: 30061898
- MacKay JL & Hammer DA Stiff substrates enhance monocytic cell capture through E-selectin but not P-selectin. Integr Biol (Camb) 8:62-72 (2016). PubMed: 26626697