Native Human Serum Albumin protein (FITC) (ab8030)
Key features and details
- Expression system: Native
- Suitable for: Dot blot, WB
Description
-
Product name
Native Human Serum Albumin protein (FITC)
See all Human Serum Albumin proteins and peptides -
Expression system
Native -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Native -
-
Species
Human -
Predicted molecular weight
69 kDa
-
-
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Description
Native Human Human Serum Albumin protein (FITC)
Specifications
Our Abpromise guarantee covers the use of ab8030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Dot blot
Western blot
-
Form
Lyophilized -
Additional notes
Fluorochrome/Protein Ratio: 1.5 moles FITC per mole of Human Albumin.
AB8030 contains Polyethylene glycol at 10 mg/mL as a stabilizer.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C long term. Avoid freeze / thaw cycle. Reconstitute for long term storage.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% Polyethylene glycol -
ReconstitutionReconstitute with 1.0 mL of deionized water
General Info
-
Alternative names
- alb
- ALBU_HUMAN
- Albumin
see all -
Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab8030 has been referenced in 9 publications.
- Sutton MN et al. RAS-Driven Macropinocytosis of Albumin or Dextran Reveals Mutation-Specific Target Engagement of RAS p.G12C Inhibitor ARS-1620 by NIR-Fluorescence Imaging. Mol Imaging Biol 24:498-509 (2022). PubMed: 34905147
- Liang X et al. Extracellular vesicles engineered to bind albumin demonstrate extended circulation time and lymph node accumulation in mouse models. J Extracell Vesicles 11:e12248 (2022). PubMed: 35879268
- Offeddu GS et al. Microphysiological endothelial models to characterize subcutaneous drug absorption. ALTEX N/A:N/A (2022). PubMed: 36533850
- Zhang SY & Mahler GJ Modelling Renal Filtration and Reabsorption Processes in a Human Glomerulus and Proximal Tubule Microphysiological System. Micromachines (Basel) 12:N/A (2021). PubMed: 34442605
- Bres EE et al. Lipoprotein receptor loss in forebrain radial glia results in neurological deficits and severe seizures. Glia 68:2517-2549 (2020). PubMed: 32579270
- Offeddu GS et al. An on-chip model of protein paracellular and transcellular permeability in the microcirculation. Biomaterials 212:115-125 (2019). PubMed: 31112823
- Offeddu GS et al. Application of Transmural Flow Across In Vitro Microvasculature Enables Direct Sampling of Interstitial Therapeutic Molecule Distribution. Small 15:e1902393 (2019). PubMed: 31497931
- Zadravec P et al. Development of Recombinant Lactococcus lactis Displaying Albumin-Binding Domain Variants against Shiga Toxin 1 B Subunit. PLoS One 11:e0162625 (2016). ELISA . PubMed: 27606705
- Homan KA et al. Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips. Sci Rep 6:34845 (2016). PubMed: 27725720