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Bioactive grade

Native human Thrombin protein (Active) (ab62452)

Submit a review Q&A (2)References (3)
SDS-PAGE - Native human Thrombin protein (Active) (ab62452)

    Key features and details

    • Expression system: Native
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Native human Thrombin protein (Active)
      See all Thrombin proteins and peptides

    • Biological activity

      This product can be used for cleavage of fusion proteins. The activity varies form lot to lot. It is typically 3800 U/mg.

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Native

    • Accession

      P00734

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Native

      • Species

        Human

    Specifications

    Our Abpromise guarantee covers the use of ab62452 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid

    • Additional notes

      Concentration varies from lot to lot but is between 5mg/ml and 10mg/ml.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

      Constituent: 50% Glycerol

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Coagulation factor II
      • Coagulation factor II thrombin
      • EC 3.4.21.5
      • F2
      • Factor II
      • Prepro coagulation factor II
      • Prothrombin
      • Prothrombin B chain
      • PT
      • RPRGL2
      • Serine protease
      • THPH1
      • THRB_HUMAN
      • Thrombin heavy chain
      see all

    • Function

      Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

    • Tissue specificity

      Expressed by the liver and secreted in plasma.

    • Involvement in disease

      Factor II deficiency
      Ischemic stroke
      Thrombophilia due to thrombin defect
      Pregnancy loss, recurrent, 2

    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.

    • Post-translational
      modifications

      The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
      N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).

    • Cellular localization

      Secreted, extracellular space.
    • Information by UniProt

    Images

    • SDS-PAGE - Native human Thrombin protein (Active) (ab62452)
      SDS-PAGE - Native human Thrombin protein (Active) (ab62452)
      Novex 4-12% Bis-Tris
      1 µg per lane
      MOPS

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab62452? Please let us know so that we can cite the reference in this datasheet.

    ab62452 has been referenced in 3 publications.

    • Schmidt C  et al. A multiparametric fluorescence assay for screening aptamer-protein interactions based on microbeads. Sci Rep 12:2961 (2022). PubMed: 35194086
    • Amick KA  et al. Plasma glycocholic acid and linoleic acid identified as potential mediators of mitochondrial bioenergetics in Alzheimer's dementia. Front Aging Neurosci 14:954090 (2022). PubMed: 36212044
    • Park HJ  et al. One-pot colorimetric detection of molecules based on proximity proteolysis reaction. Biosens Bioelectron 188:113349 (2021). PubMed: 34030090

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    ab62452
    Active full length protein
    How is the protein prepared? Is this native purified protein?
    Cleaved protein is active which would not be full length.

    Read More
    Answer


    I can confirm this is native fully active protein. The Prothrombin is purified directly from plasma. The Prothrombin is then activated to Thrombin via a Prothrombinase reaction.

    PROTEOLYTIC ACTIVATION OF PROTHROMBIN The serine protease α-thrombin is produced by proteolytic activation of the zymogen, prothrombin. The enzyme complex, prothrombinase, catalyzes the proteolysis of two peptide bonds in prothrombin, which gives rise to an NH2-terminal derived F1.2 region and the heterodimer, alpha-thrombin. Alpha-thrombin is composed of an A chain (Mr=6000) which is covalently linked to a B chain (Mr=31,000) through a single disulfide bond.

    I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question

    Is the Thrombin protein ab62452 in it's active form?
    Do you know the unit amount supplied within the 100ug included?

    Read More
    Answer


    I have checked with the lab and this protein is indeed active.

    For lot GR147433 currently in stock, we have determined the activity to be 3034 U/mg therefore 303.4 U per 100 ug.

    Read More

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