Native human Thrombin protein (Active) (ab62452)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Native human Thrombin protein (Active)
See all Thrombin proteins and peptides -
Biological activity
This product can be used for cleavage of fusion proteins. The activity varies form lot to lot. It is typically 3800 U/mg.
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Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab62452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Concentration varies from lot to lot but is between 5mg/ml and 10mg/ml.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituent: 50% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Coagulation factor II
- Coagulation factor II thrombin
- EC 3.4.21.5
see all -
Function
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
Cellular localization
Secreted, extracellular space. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab62452 has been referenced in 3 publications.
- Schmidt C et al. A multiparametric fluorescence assay for screening aptamer-protein interactions based on microbeads. Sci Rep 12:2961 (2022). PubMed: 35194086
- Amick KA et al. Plasma glycocholic acid and linoleic acid identified as potential mediators of mitochondrial bioenergetics in Alzheimer's dementia. Front Aging Neurosci 14:954090 (2022). PubMed: 36212044
- Park HJ et al. One-pot colorimetric detection of molecules based on proximity proteolysis reaction. Biosens Bioelectron 188:113349 (2021). PubMed: 34030090