Recombinant Anti-LASP1 antibody [EPR11221] (ab156872)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11221] to LASP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-LASP1 antibody [EPR11221]
See all LASP1 primary antibodies -
Description
Rabbit monoclonal [EPR11221] to LASP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human LASP1 (C terminal).
-
Positive control
- Recombinant Human LASP1 protein (ab114888) can be used as a positive control in WB. SK-OV-3, A431, U87-MG and 293T cell lysates, and A431 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11221 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
|
|
ICC/IF |
1/100 - 1/250.
|
Notes |
---|
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa. |
ICC/IF
1/100 - 1/250. |
Target
-
Function
Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 3927 Human
- Entrez Gene: 16796 Mouse
- Entrez Gene: 29278 Rat
- Omim: 602920 Human
- SwissProt: Q14847 Human
- SwissProt: Q61792 Mouse
- SwissProt: Q99MZ8 Rat
- Unigene: 728766 Human
see all -
Alternative names
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
see all
Images
-
All lanes : Anti-LASP1 antibody [EPR11221] (ab156872) at 1/10000 dilution
Lane 1 : SK-OV-3 cell lysates
Lane 2 : A431 cell lysate
Lane 3 : U87-MG cell lysates
Lane 4 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 30 kDa -
Immunofluorescent analysis of A431 cells labeling LASP1 with ab156872 at 1/100.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab156872 has been referenced in 7 publications.
- Han P et al. Circular RNA hsa_circ_0003892 promotes the development of papillary thyroid carcinoma by regulating the miR-326/LASP1 axis. Histol Histopathol N/A:18546 (2022). PubMed: 36394255
- Tang R et al. Circular RNA circ_0006948 Promotes Esophageal Squamous Cell Carcinoma Progression by Regulating microRNA-3612/LASP1 Axis. Dig Dis Sci N/A:N/A (2021). PubMed: 34024023
- Jin H et al. Long non-coding RNA H19 regulates LASP1 expression in osteosarcoma by competitively binding to miR-29a-3p. Oncol Rep 46:N/A (2021). PubMed: 34328197
- Zhang Z et al. Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway. Biosci Rep 39:N/A (2019). PubMed: 30988074
- Balahmar RM et al. Identification and characterisation of NANOG+/ OCT-4high/SOX2+ doxorubicin-resistant stem-like cells from transformed trophoblastic cell lines. Oncotarget 9:7054-7065 (2018). PubMed: 29467949
- Zhao H et al. LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway. Oncol Lett 15:6839-6844 (2018). PubMed: 29731863
- Pan X et al. MicroRNA-206 inhibits the viability and migration of medulloblastoma cells by targeting LIM and SH3 protein 1. Exp Ther Med 14:3894-3900 (2017). PubMed: 29042998