Recombinant Cynomolgus monkey SIRP alpha protein (Tagged) (Biotin) (ab271742)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Cynomolgus monkey SIRP alpha protein (Tagged) (Biotin)
See all SIRP alpha proteins and peptides -
Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Cynomolgus monkey -
Sequence
EEELQVIQPE KSVSVAAGDS ATLNCTVSSL IPVGPIQWFR GAGPGRELIY NLKEGHFPRV TAVSDPTKRN NMDFSIRISN ITPADAGTYY CVKFRKGSPD VELKSGAGTE LSVRAKPSAP VVSGPAVRAT AEHTVSFTCE SHGFSPRDIT LKWFKNGNEL SDVQTNVDPA GKSVSYSIRS TARVLLTRRD VHSQVICEVA HVTLQGDPLR GTANLSEAIR VPPFLEVTQQ SMRADNQVNV TCQVTKFYPQ RLQLTWLENG NVSRTEMASA LPENKDGTYN WTSWLLVNVS AHRDDVKLTC QVEHDGQPAV NKSFSVKVSA HPKEQGSNTA AENTGTNERN -
Predicted molecular weight
42 kDa -
Amino acids
31 to 370 -
Tags
His tag C-Terminus , Avi tag C-Terminus
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Conjugation
Biotin
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab271742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
Enzymatically biotin-labeled using Avi-tag™ technology
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Signal regulatory protein alpha type 1
- Bit
- Brain Ig like molecule with tyrosine based activation motifs
see all -
Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
Tissue specificity
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Cynomolgus monkey SIRP alpha protein (Tagged) (Biotin) (ab271742)
SDS-PAGE analysis of 4 µg ab271742.
This protein is heavily glycosylated, resulting in apparent higher molecular weight.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab271742 has not yet been referenced specifically in any publications.