Recombinant E. coli BirA protein (ab198449)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 78% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Conjugation, Purification, SDS-PAGE
Description
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Product name
Recombinant E. coli BirA protein
See all BirA proteins and peptides -
Biological activity
BirA (16 µg/mL) completely biotinlabels 10 µM of Avi-tag substrate at 30°C for 30 minutes in reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM dBiotin.
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Purity
>= 78 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLVPRGSKDNTVPLKLIALLANGEFHSGEQL GETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQ LDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFS PFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPN DLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWIT LQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRP VKLIIGDKEIFGISRGIDKQGALLLE QDGIIKPWMGGEISLRSAEK -
Predicted molecular weight
61 kDa including tags -
Amino acids
2 to 321 -
Tags
GST tag N-Terminus
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Description
Recombinant E. coli BirA protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Conjugation
Purification
SDS-PAGE
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Form
Liquid -
Additional notes
Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.49% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Bifunctional protein birA
- Bio R
- BioR
see all -
Relevance
BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.
Images
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Conjugation - Recombinant E. coli BirA protein (ab198449)
Image A: SDS-PAGE with Coomassie blue showing:
Lane 1: Unbiotinylated BirA
Lane 2: ab198449
Lane 3: BRD4-Avi-biotin control
Lane 4: Protein markers
Image B. Shows the corresponding Western blot probed with Streptavidin-HRP and developed with ECL reagent.
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SDS-PAGE - Recombinant E. coli BirA protein (ab198449)10% SDS-PAGE with Coomassie staining: 4 µg ab198449.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab198449 has been referenced in 1 publication.
- Zheng Q et al. A bifunctional molecule-assisted synthesis of mimics for use in probing the ubiquitination system. Nat Protoc 18:530-554 (2023). PubMed: 36323865