Recombinant E. coli groEL protein (ab113176)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: WB, EIA, Functional Studies, ELISA
Description
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Product name
Recombinant E. coli groEL protein
See all groEL proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab113176 is purified by multi-step chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITK DGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIIT EGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTIS ANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLS PYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIA EDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVI SEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQ QIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALH ATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPL RQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTR SALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM -
Predicted molecular weight
57 kDa -
Amino acids
2 to 548 -
Additional sequence information
Strain K12.
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Description
Recombinant E. coli groEL protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab113176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Enzyme Immunoassay
Functional Studies
ELISA
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 0.58% Sodium chloride, 0.79% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.1% Magnesium chloride
General Info
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Alternative names
- 60 kDa chaperonin
- groEL protein
- groL
see all -
Relevance
The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner. -
Cellular localization
Cytoplasmic
Images
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SDS-PAGE - Recombinant E. coli groEL protein (ab113176)
SDS-PAGE analysis of ab113176.
Lane 1: MW marker
Lane 2: 0.5 μg of ab113176 (groEL protein)
Lane 3: 1 μg of ab113176 (groEL protein)
Lane 4: 2 μg of ab113176 (groEL protein)
Lane 5: 5 μg of ab113176 (groEL protein)
Detected by Coomassie stain. -
Western blot - Recombinant E. coli groEL protein (ab113176)Western blot analysis using ab113176.
Lane 1: MW marker
Lane 2: 100 ng of Recombinant E. coli groEL protein (ab113176) probed with an anti-groEL monoclonal antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab113176 has not yet been referenced specifically in any publications.