Recombinant E. coli Multi Tag protein (ab36791)
Key features and details
- Expression system: Escherichia coli
- Suitable for: WB
Description
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Product name
Recombinant E. coli Multi Tag protein -
Biological activity
This protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Predicted molecular weight
45 kDa
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Description
Recombinant E. coli Multi Tag protein
Specifications
Our Abpromise guarantee covers the use of ab36791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
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Form
Liquid -
Additional notes
This protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis.
This product is a bacterial lysate provided in1 x reduced SDS-PAGE loading buffer. It is effective as a positive control for epitope tag antibodies in immunoblotting.
For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µl. It is recommended that the reagent be titrated for optimal performance for each application.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 6.80
Constituents: 0.76% Tris, 2% Sodium lauryl sulfate, 10% Glycerol, 0.01% Bromophenol blue
Images
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Western blot - Recombinant E. coli Multi Tag protein (ab36791)All lanes : Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µl
Lane 1 : HA-HRP
Lane 2 : Myc-HRP
Lane 3 : Glu-Glu
Lane 4 : GST
Lane 5 : His
Lane 6 : HSV
Lane 7 : T7
Lane 8 : V5
Lane 9 : VSV
Lane 10 : S-Tag
Lane 11 : FLAG-HRP
Predicted band size: 45 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab36791 has been referenced in 1 publication.
- Trofka A et al. The Her7 node modulates the network topology of the zebrafish segmentation clock via sequestration of the Hes6 hub. Development 139:940-7 (2012). WB . PubMed: 22278920