Recombinant E. coli SSB protein (ab123224)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant E. coli SSB protein
See all SSB proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Predicted molecular weight
19 kDa -
Amino acids
2 to 178
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Description
Recombinant E. coli SSB protein
Specifications
Our Abpromise guarantee covers the use of ab123224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
pH: 6.5
Constituents: 0.02% DTT, 0.32% Tris HCl, 0.03% EDTA, 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride
General Info
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Alternative names
- Autoantigen La
- La
- La autoantigen
see all -
Function
Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. -
Sequence similarities
Contains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
The N-terminus is blocked. -
Cellular localization
Nucleus. - Information by UniProt
Images
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SDS-PAGE - Recombinant E. coli SSB protein (ab123224)SDS-PAGE of ab123224
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab123224 has been referenced in 4 publications.
- Kretov DA et al. YB-1, an abundant core mRNA-binding protein, has the capacity to form an RNA nucleoprotein filament: a structural analysis. Nucleic Acids Res N/A:N/A (2019). PubMed: 30605522
- Nguyen HD et al. Functions of Replication Protein A as a Sensor of R Loops and a Regulator of RNaseH1. Mol Cell 65:832-847.e4 (2017). Functional Studies . PubMed: 28257700
- Krauss G et al. Escherichia coli single-strand deoxyribonucleic acid binding protein: stability, specificity, and kinetics of complexes with oligonucleotides and deoxyribonucleic acid. Biochemistry 20:5346-52 (1981). PubMed: 7028102
- Weiner JH et al. The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication. J Biol Chem 250:1972-80 (1975). PubMed: 1090613