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  1. Link

    products/proteins-peptides/recombinant-hepatitis-c-virus-ns3-protein-ab49022.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus Hepatitis A/C/E/G
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Recombinant Hepatitis C Virus NS3 protein (ab49022)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: GST tag N-Terminus
  • Suitable for: ELISA, Flow Cyt, SDS-PAGE, WB

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Description

  • Product name

    Recombinant Hepatitis C Virus NS3 protein
    See all Hepatitis C Virus NS3 proteins and peptides
  • Biological activity

    Strongly reacts with human HCV positive serum.
  • Purity

    > 95 % SDS-PAGE.
    Purity of proteins is evaluated by SDS-PAGE
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino acids

      1192 to 1456
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      HCV polyprotein (genotype 1B)

Specifications

Our Abpromise guarantee covers the use of ab49022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Flow Cytometry

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

    Strongly reacts with human HCV positive serum.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.2
    Constituents: 0.2% Triton-X-100, 9% Urea, 0.395% Tris HCl, 50% Glycerol (glycerin, glycerine)

General Info

  • Alternative names

    • Hepatitis C Virus NS3
    • NS3
    • NS3P
    • p70
    • Serine protease/NTPase/helicase
    see all
  • Relevance

    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localization

    Endoplasmic reticulum membrane

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab49022? Please let us know so that we can cite the reference in this datasheet.

ab49022 has been referenced in 1 publication.

  • Subramaniam AB  et al. Metal-amplified Density Assays, (MADAs), including a Density-Linked Immunosorbent Assay (DeLISA). Lab Chip 15:1009-22 (2015). PubMed: 25474561

Customer reviews and Q&As

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Question

Dear Colleagues, My customer is interested in one of the products: ab49022, ab49024, ab49025. She would like to know for how many standard Flow Cyt applications they are dedicated? I would be grateful for your help and quick response. Best regards,  

Read More

Abcam community

Verified customer

Asked on Oct 11 2011

Answer

Thank you for your enquiry and your interest in our products. Generally, one vial of 100 ug of protein of these products (ab49022, ab49024, ab49025) typically can give 50 to 100 tests depending on the format. More tests can be performed for ELISA and less for lateral flow. However, all depends on the customers’ needs. I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on Oct 11 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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