For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    By product type
    Proteins and Peptides
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/proteins-peptides/recombinant-human-ace2-mutated-h374n--h378n-protein-fc-chimera-ab273885.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus
Share by email

Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)
  • SDS-PAGE - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)
  • HPLC - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% Affinity purified
  • Endotoxin level: < 1.000 Eu/mg
  • Tags: Fc tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC, ELISA

You may also be interested in

ELISA
Product image
Human ACE2 ELISA Kit (ab235649)
Pair
Product image
Human ACE2 Antibody Pair - BSA and Azide free (ab244135)
Protein
Product image
Recombinant Human ACE2 protein (Fc Chimera) (ab273687)

View more associated products

Description

  • Product name

    Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera)
    See all ACE2 proteins and peptides
  • Purity

    > 95 % Affinity purified.

  • Endotoxin level

    < 1.000 Eu/mg
  • Expression system

    HEK 293 cells
  • Accession

    Q9BYF1
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADGG GGSGGGGSDK THTCPPCPAP EAAGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK
    • Predicted molecular weight

      190 kDa including tags
    • Amino acids

      18 to 615
    • Modifications

      mutated H374N + H378N
    • Tags

      Fc tag C-Terminus
    • Additional sequence information

      Fc domain: Human IgG1 containing silencing LALA mutations.

Associated products

  • Related Products

    • Recombinant Human ACE2 protein (Fc Chimera) (ab273687)

Specifications

Our Abpromise guarantee covers the use of ab273885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    HPLC

    ELISA

  • Form

    Liquid
  • Additional notes

    Recombinant Human ACE2 (mutated H374 N + H378 N) protein (Fc Chimera) (ab273885) is an inactivated form of ACE2 generated from ACE2-Ig by altering the codons of ACE2 active-site histidines 374 and 378 to those of asparagines.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C.

    Constituent: PBS

General Info

  • Alternative names

    • ACE 2
    • ACE related carboxypeptidase
    • ACE-related carboxypeptidase
    • ACE2
    • ACE2_HUMAN
    • ACEH
    • Angiotensin converting enzyme 2
    • Angiotensin converting enzyme homolog
    • Angiotensin converting enzyme like protein
    • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2
    • Angiotensin I converting enzyme 2
    • Angiotensin-converting enzyme homolog
    • DKFZP434A014
    • EC 3.4.17
    • metalloprotease MPROT 15
    • Metalloprotease MPROT15
    • OTTHUMP00000022963
    • Processed angiotensin-converting enzyme 2
    see all
  • Function

    Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • Tissue specificity

    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • Sequence similarities

    Belongs to the peptidase M2 family.
  • Post-translational
    modifications

    N-glycosylation on Asn-90 may limit SARS infectivity.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q9BYF1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • ELISA - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)
    ELISA - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)

    RBD Fc binding to immobilized ACE2 Fc fusion proteins. 96-well plates were coated with Recombinant Human ACE2 protein (Fc Chimera) (ab273687) and ab273885 at 5 µg/ml. The SARS-CoV-2 RBD Fc fusion protein conjugated to HRP was titrated on a 3-fold serial dilution starting at 10 µg/ml.

  • SDS-PAGE - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)
    SDS-PAGE - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)

    SDS-PAGE analysis of 2.5 µg ab273885.

    Lane 1: Non-reduced.

    Lane 2: Reduced.

  • HPLC - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)
    HPLC - Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) (ab273885)

    HPLC analysis of ab273885.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab273885? Please let us know so that we can cite the reference in this datasheet.

ab273885 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab273885.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.