Recombinant Human ACE2 protein (Fc Chimera) (ab273687)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% Affinity purified
- Endotoxin level: < 1.000 Eu/mg
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, HPLC, ELISA
Description
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Product name
Recombinant Human ACE2 protein (Fc Chimera)
See all ACE2 proteins and peptides -
Purity
> 95 % Affinity purified. -
Endotoxin level
< 1.000 Eu/mg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADGG GGSGGGGSDK THTCPPCPAP EAAGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK -
Predicted molecular weight
190 kDa including tags -
Amino acids
18 to 615 -
Tags
Fc tag C-Terminus -
Additional sequence information
Fc domain: Human IgG1 containing silencing LALA mutations.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab273687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
HPLC
ELISA
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C.
Constituent: PBS
General Info
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Alternative names
- ACE 2
- ACE related carboxypeptidase
- ACE-related carboxypeptidase
see all -
Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
Images
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ELISA - Recombinant Human ACE2 protein (Fc Chimera) (ab273687)
RBD Fc binding to immobilized ACE2 Fc fusion proteins. 96-well plates were coated with ab273687 and Recombinant Human ACE2 (mutated H374 N + H378 N) protein (Fc Chimera) (ab273885) at 5 µg/ml. The SARS-CoV-2 RBD Fc fusion protein conjugated to HRP was titrated on a 3-fold serial dilution starting at 10 µg/ml.
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ELISA - Recombinant Human ACE2 protein (Fc Chimera) (ab273687)ab273687 binding to immobilized SARS-CoV-2 RBD Fc fusion protein. 96-well plate was coated with the SARS-CoV-2 RBD Fc fusion protein at 5 µg/ml. ab273687 was titrated on a 3-fold serial dilution starting at 10 µg/ml.
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SDS-PAGE - Recombinant Human ACE2 protein (Fc Chimera) (ab273687)SDS-PAGE analysis of 2.5 µg ab273687.
Lane 1: Non-reduced.
Lane 2: Reduced.
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HPLC - Recombinant Human ACE2 protein (Fc Chimera) (ab273687)HPLC analysis of ab273687.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (6)
ab273687 has been referenced in 6 publications.
- Ma Y et al. SARS-CoV-2 Spike Stem Protein Nanoparticles Elicited Broad ADCC and Robust Neutralization against Variants in Mice. Small 18:e2200836 (2022). PubMed: 35607768
- Sarker H et al. Glucocorticoids Bind to SARS-CoV-2 S1 at Multiple Sites Causing Cooperative Inhibition of SARS-CoV-2 S1 Interaction With ACE2. Front Immunol 13:906687 (2022). PubMed: 35784352
- Polyiam K et al. Immunodominant linear B cell epitopes in the spike and membrane proteins of SARS-CoV-2 identified by immunoinformatics prediction and immunoassay. Sci Rep 11:20383 (2021). PubMed: 34650130
- Rattanapisit K et al. Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana. Sci Rep 10:17698 (2020). PubMed: 33077899
- Carpinteiro A et al. Pharmacological Inhibition of Acid Sphingomyelinase Prevents Uptake of SARS-CoV-2 by Epithelial Cells. Cell Rep Med 1:100142 (2020). PubMed: 33163980
- Edwards MJ et al. Sphingosine prevents binding of SARS-CoV-2 spike to its cellular receptor ACE2. J Biol Chem 295:15174-15182 (2020). PubMed: 32917722
