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Recombinant human ALDH3A1 protein (ab93463)

Submit a review Q&A (1)
SDS-PAGE - Recombinant human ALDH3A1 protein (ab93463)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant human ALDH3A1 protein

    • Biological activity

      Specific activity is >5 units/ml and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols. Specific activity is >5 units/ml and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols.

    • Purity

      > 95 % SDS-PAGE.
      ab93463 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MSKISEAVKR ARAAFSSGRT RPLQFRIQQL EALQRLIQEQ EQELVGALAA DLHKNEWNAY YEEVVYVLEE IEYMIQKLPE WAADEPVEKT PQTQQDELYI HSEPLGVVLV IGTWNYPFNL TIQPMVGAIA AGNAVVLKPS ELSENMASLL ATIIPQYLDK DLYPVINGGV PETTELLKER FDHILYTGST GVGKIIMTAA AKHLTPVTLE LGGKSPCYVD KNCDLDVACR RIAWGKFMNS GQTCVAPDYI LCDPSIQNQI VEKLKKSLKE FYGEDAKKSR DYGRIISARH FQRVMGLIEG QKVAYGGTGD AATRYIAPTI LTDVDPQSPV MQEEIFGPVL PIVCVRSLEE AIQFINQREK PLALYMFSSN DKVIKKMIAE TSSGGVAAND VIVHITLHSL PFGGVGNSGM GSYHGKKSFE TFSHRRSCLV RPLMNDEGLK VRYPPSPAKM TQH

    Specifications

    Our Abpromise guarantee covers the use of ab93463 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Acetaldehyde dehydrogenase 3
      • AHD 4
      • AHD C
      • AHD4
      • AHDC
      • AL3A1_HUMAN
      • Aldehyde dehydrogenase
      • Aldehyde dehydrogenase 3
      • Aldehyde dehydrogenase 3 family member A1
      • Aldehyde dehydrogenase 3A1
      • Aldehyde dehydrogenase class 3
      • Aldehyde dehydrogenase dimeric NADP preferring
      • Aldehyde dehydrogenase family 3 member A1
      • Aldehyde dehydrogenase family 3 subfamily A1
      • Aldehyde dehydrogenase family 3, subfamily A, member 1
      • Aldehyde dehydrogenase isozyme 3
      • Aldehyde dehydrogenase type III
      • ALDH 3
      • ALDH 3A1
      • ALDH III
      • ALDH, stomach type
      • ALDH3
      • ALDH3 A1
      • ALDH3A 1
      • Aldh3a1
      • ALDHIII
      • dimeric NADP-preferring
      • MGC10406
      • Stomach aldehyde dehydrogenase
      • Tumor associated aldehyde dehydrogenase tumor ALDH
      see all

    • Function

      ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.

    • Tissue specificity

      High levels in stomach, esophagus and lung; low level in the liver and kidney.

    • Sequence similarities

      Belongs to the aldehyde dehydrogenase family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human ALDH3A1 protein (ab93463)
      SDS-PAGE - Recombinant human ALDH3A1 protein (ab93463)
      15% SDS-PAGE analysis of ab93463 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab93463? Please let us know so that we can cite the reference in this datasheet.

    ab93463 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I do not understand the meaning of "specific activity is < 1 units/ml".
    When we use it, we need assess the activity again. As we lack of
    experience of ALDH activity assay, could you let us know the protocol you
    used for the assessment? Thanks again for the help in advance.

    Read More
    Answer

    Thank you for your reply. The specific information activity for these products is as follows:


    ab87415: Specific activity is > 0.14 units/ml

    ab93463 and ab117154: Specific activity is > 0.5 units/ml

    The protocol used to measure the specific activity is:

    Activity Assay

    1) Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA.
    2) Equilibrate to 25C and monitor the A340nm until value is constant using a spectrophotometer.
    3) Add 5ug of recombinant ALDH2 into reaction mixture and mix immediately.
    4) Record the increase in A340nm for 5 minutes



    I hope this helps, please let me know if you need any additional information.

    Read More

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