Recombinant Human alpha smooth muscle Actin protein (ab134555)

Submit a review Submit a question References (1)
SDS-PAGE - Recombinant Human alpha smooth muscle Actin protein (ab134555)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human alpha smooth muscle Actin protein

    • Purity

      > 85 % SDS-PAGE.
      ab134555 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P62736

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSHMEEEDSTALVCDNGSGLCKAGFAGDD APRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI ITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFE TFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAI MRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFEN EMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIH ETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTM KIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

      • Predicted molecular weight

        44 kDa including tags

      • Amino acids

        3 to 377

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab134555 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • a actin
      • AAT6
      • ACTA_HUMAN
      • ACTA2
      • Actin alpha 2 smooth muscle aorta
      • Actin aortic smooth muscle
      • Actin, aortic smooth muscle
      • ACTSA
      • ACTVS
      • Alpha 2 actin
      • Alpha actin 2
      • Alpha cardiac actin
      • alpha sma
      • Alpha-actin-2
      • Cell growth inhibiting gene 46 protein
      • Cell growth-inhibiting gene 46 protein
      • GIG46
      • Growth inhibiting gene 46
      • MYMY5
      see all

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

    • Involvement in disease

      Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.

    • Sequence similarities

      Belongs to the actin family.

    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human alpha smooth muscle Actin protein (ab134555)
      SDS-PAGE - Recombinant Human alpha smooth muscle Actin protein (ab134555)
      15% SDS-PAGE analysis of 3 µg ab134555.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab134555? Please let us know so that we can cite the reference in this datasheet.

    ab134555 has been referenced in 1 publication.

    • Gordon O  et al. a-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster. Elife 7:N/A (2018). PubMed: 30260317

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab134555.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com