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Recombinant Human AMPK alpha 1 protein (ab202156)

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SDS-PAGE - Recombinant Human AMPK alpha 1 protein (ab202156)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human AMPK alpha 1 protein

    • Purity

      > 85 % SDS-PAGE.
      ab202156 was purified by conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q13131

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSMRRLSSWRKMATAEKQKHDGRVKIGHY ILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQ NLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKES RRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSD GEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDD DHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHE WF

      • Predicted molecular weight

        34 kDa including tags

      • Amino acids

        1 to 279

      • Tags

        His tag N-Terminus

      • Additional sequence information

        NP_006242.

    Specifications

    Our Abpromise guarantee covers the use of ab202156 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • 5 AMP activated protein kinase alpha 1catalytic subunit
      • 5 AMP activated protein kinase catalytic alpha 1 chain
      • 5' AMP activated protein kinase catalytic subunit alpha 1
      • 5'-AMP-activated protein kinase catalytic subunit alpha-1
      • AAPK1
      • AAPK1_HUMAN
      • ACACA kinase
      • acetyl CoA carboxylase kinase
      • AI194361
      • AI450832
      • AL024255
      • AMP -activate kinase alpha 1 subunit
      • AMP-activate kinase alpha 1 subunit
      • AMP-activated protein kinase, catalytic, alpha -1
      • AMPK
      • AMPK 1
      • AMPK alpha 1
      • AMPK alpha 1 chain
      • AMPK subunit alpha 1
      • AMPK subunit alpha-1
      • AMPK1
      • AMPKa1
      • AMPKalpha1
      • C130083N04Rik
      • cb116
      • EC 2.7.11.1
      • HMG CoA reductase kinase
      • HMGCR kinase
      • hormone sensitive lipase kinase
      • Hydroxymethylglutaryl CoA reductase kinase
      • im:7154392
      • kinase AMPK alpha1
      • MGC33776
      • MGC57364
      • OTTHUMP00000161795
      • OTTHUMP00000161796
      • PRKAA 1
      • PRKAA1
      • Protein kinase AMP activated alpha 1 catalytic subunit
      • SNF1-like protein AMPK
      • SNF1A
      • Tau protein kinase PRKAA1
      • wu:fa94c10
      see all

    • Function

      Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human AMPK alpha 1 protein (ab202156)
      SDS-PAGE - Recombinant Human AMPK alpha 1 protein (ab202156)

      15% SDS-PAGE analysis of ab202156 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab202156? Please let us know so that we can cite the reference in this datasheet.

    ab202156 has not yet been referenced specifically in any publications.

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