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    products/proteins-peptides/recombinant-human-beta-actin-protein-his-tag-ab240844.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Recombinant Human beta Actin protein (His tag) (ab240844)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human beta Actin protein (His tag) (ab240844)
  • Mass Spectrometry - Recombinant Human beta Actin protein (His tag) (ab240844)
  • Mass Spectrometry - Recombinant Human beta Actin protein (His tag) (ab240844)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, MS

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Description

  • Product name

    Recombinant Human beta Actin protein (His tag)
    See all beta Actin proteins and peptides
  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

    P60709
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      PVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGI VMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFT TTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIG NERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSG GTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST FQQMWISKQEYDESGPSIVHRKCF
    • Predicted molecular weight

      35 kDa including tags
    • Amino acids

      102 to 375
    • Tags

      His tag N-Terminus
    • Additional sequence information

      N-terminal 6xHis-tagged.

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)

Specifications

Our Abpromise guarantee covers the use of ab240844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.2
    Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

General Info

  • Alternative names

    • A26C1A
    • A26C1B
    • ACTB
    • ACTB_HUMAN
    • Actin beta
    • Actin cytoplasmic 1
    • Actin, cytoplasmic 1, N-terminally processed
    • Actx
    • b actin
    • b-actin
    • Beta cytoskeletal actin
    • Beta-actin
    • BRWS1
    • E430023M04Rik
    • Melanoma X actin
    • MGC128179
    • PS1TP5 binding protein 1
    • PS1TP5BP1
    see all
  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Human beta Actin protein (His tag) (ab240844)
    SDS-PAGE - Recombinant Human beta Actin protein (His tag) (ab240844)

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab240844.

  • Mass Spectrometry - Recombinant Human beta Actin protein (His tag) (ab240844)
    Mass Spectrometry - Recombinant Human beta Actin protein (His tag) (ab240844)

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab240844 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) beta Actin.

  • Mass Spectrometry - Recombinant Human beta Actin protein (His tag) (ab240844)
    Mass Spectrometry - Recombinant Human beta Actin protein (His tag) (ab240844)

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab240844 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) beta Actin.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab240844? Please let us know so that we can cite the reference in this datasheet.

ab240844 has not yet been referenced specifically in any publications.

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