Recombinant Human beta Actin protein (His tag) (ab240844)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human beta Actin protein (His tag)
See all beta Actin proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
PVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGI VMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFT TTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIG NERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSG GTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST FQQMWISKQEYDESGPSIVHRKCF -
Predicted molecular weight
35 kDa including tags -
Amino acids
102 to 375 -
Tags
His tag N-Terminus -
Additional sequence information
N-terminal 6xHis-tagged.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab240844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- A26C1A
- A26C1B
- ACTB
see all -
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human beta Actin protein (His tag) (ab240844)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab240844.
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Mass Spectrometry - Recombinant Human beta Actin protein (His tag) (ab240844)Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab240844 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) beta Actin.
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Mass Spectrometry - Recombinant Human beta Actin protein (His tag) (ab240844)Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab240844 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) beta Actin.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab240844 has not yet been referenced specifically in any publications.