Recombinant Human Bim protein (ab112387)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, ELISA, WB
Description
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Product name
Recombinant Human Bim protein
See all Bim proteins and peptides -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQDRSPAPMSC DKSTQTPSPPCQAFNHYLSAMVVILEDIGDLSLCFGFIFTGLDLYGHHHS QDTEQLNHKDFS -
Predicted molecular weight
39 kDa including tags -
Amino acids
1 to 112 -
Tags
GST tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab112387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
ELISA
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
Glutathione is reduced
General Info
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Alternative names
- BCL2 like 11
- B2L11_HUMAN
- BAM
see all -
Function
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis. -
Tissue specificity
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
The BH3 motif is required for Bcl-2 binding and cytotoxicity. -
Cellular localization
Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Bim protein (ab112387)12.5% SDS-PAGE analysis of ab112387. Stained with Coomassie Blue
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab112387 has been referenced in 1 publication.
- Cheng Mm W et al. Role of the mTOR Signalling Pathway in Human Sepsis-Induced Myocardial Dysfunction. Can J Cardiol 35:875-883 (2019). PubMed: 31292086
