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    products/proteins-peptides/recombinant-human-blnk-protein-ab104120.pdf

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Immunology Adaptive Immunity B Cells Non-CD
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Recombinant Human BLNK protein (ab104120)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human BLNK protein (ab104120)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

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    Description

    • Product name

      Recombinant Human BLNK protein
      See all BLNK proteins and peptides
    • Purity

      > 90 % SDS-PAGE.
      Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
    • Expression system

      Escherichia coli
    • Accession

      Q8WV28
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHH SSGLVPRGSH MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVS
      • Predicted molecular weight

        53 kDa including tags
      • Amino acids

        1 to 456
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-BLNK antibody [Y491] (ab32418)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-BLNK (phospho Y96) antibody (ab73204)
      • Anti-BLNK antibody (ab73706)

    Specifications

    Our Abpromise guarantee covers the use of ab104120 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.00174% PMSF, 0.0154% DTT, 0.316% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • AGM4
      • B cell adapter containing SH2 domain protein
      • B cell adapter containing Src homology 2 domain protein
      • B cell adaptor containing SH2 domain
      • B cell linker
      • B cell linker protein
      • B cell-specific adaptor protein
      • B-cell activation
      • B-cell adapter containing a SH2 domain protein
      • B-cell adapter containing a Src homology 2 domain protein
      • B-cell linker protein
      • BASH
      • Bca
      • BLNK
      • BLNK s
      • BLNK_HUMAN
      • Cytoplasmic adapter protein
      • Ly 57
      • Ly57
      • Lymphocyte antigen 57
      • Lyw 57
      • Lyw57
      • MGC111051
      • SH2 domain-containing leukocyte protein, 65-KD
      • SLP 65
      • SLP-65
      • SLP65
      • Src homology [SH2] domain-containing leukocyte protein of 65 kD
      • Src homology 2 domain containing leukocyte protein of 65 kDa
      • Src homology 2 domain-containing leukocyte protein of 65 kDa
      see all
    • Function

      Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
    • Tissue specificity

      Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
    • Involvement in disease

      Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
    • Sequence similarities

      Contains 1 SH2 domain.
    • Post-translational
      modifications

      Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
    • Cellular localization

      Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
    • Target information above from: UniProt accession Q8WV28 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human BLNK protein (ab104120)
      SDS-PAGE - Recombinant Human BLNK protein (ab104120)
      15% SDS-PAGE analysis of ab104120 (3 µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab104120? Please let us know so that we can cite the reference in this datasheet.

    ab104120 has not yet been referenced specifically in any publications.

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