Recombinant Human Calreticulin protein (ab91577)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human Calreticulin protein
See all Calreticulin proteins and peptides -
Purity
> 80 % SDS-PAGE.
Purity: > 80 % full-length protein as determined by Western blot analyses -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
His tag N-Terminus -
Additional sequence information
His-tag on N-terminal with thrombin cleavage site.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab91577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.056% Calcium chloride, 5% Glycerol (glycerin, glycerine), 0.877% Sodium chloride, 0.606% Tris
General Info
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Alternative names
- Autoantigen RO
- CALR
- CALR protein
see all -
Function
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. -
Sequence similarities
Belongs to the calreticulin family. -
Domain
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
Cellular localization
Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Calreticulin protein (ab91577)SDS-PAGE Analysis of ab91577 stained with Coomassie Blue.
Lane 1: MW Marker
Lane 2: 0.5 µg purified Calreticulin
Lane 3: 1 µg purified Calreticulin
Lane 4: 2 µg purified Calreticulin
Lane 5: 5 µg purified Calreticulin -
Western blot - Recombinant Human Calreticulin protein (ab91577)All lanes : Recombinant Human Calreticulin protein (ab91577) at 1/1000 dilution
Lane 1 : MW Marker
Lane 2 : 50ng Calreticulin
Lane 3 : 100ng Calreticulin -
Western blot - Recombinant Human Calreticulin protein (ab91577)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab91577 has been referenced in 2 publications.
- Chen B et al. Calreticulin Binds to Fas Ligand and Inhibits Neuronal Cell Apoptosis Induced by Ischemia-Reperfusion Injury. Biomed Res Int 2015:895284 (2015). PubMed: 26583143
- Hughes BG et al. MMP-2 is localized to the mitochondria-associated membrane of the heart. Am J Physiol Heart Circ Physiol 306:H764-70 (2014). PubMed: 24375642