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    products/proteins-peptides/recombinant-human-calreticulin-protein-ab91577.pdf

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Recombinant Human Calreticulin protein (ab91577)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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SDS-PAGE - Recombinant Human Calreticulin protein (ab91577)
  • Western blot - Recombinant Human Calreticulin protein (ab91577)
  • Western blot - Recombinant Human Calreticulin protein (ab91577)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 80% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: WB, SDS-PAGE

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Description

  • Product name

    Recombinant Human Calreticulin protein
    See all Calreticulin proteins and peptides
  • Purity

    > 80 % SDS-PAGE.
    Purity: > 80 % full-length protein as determined by Western blot analyses
  • Expression system

    Escherichia coli
  • Accession

    P27797
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Tags

      His tag N-Terminus
    • Additional sequence information

      His-tag on N-terminal with thrombin cleavage site.

Associated products

  • Related Products

    • Anti-Calreticulin antibody (ab15607)
    • Anti-Calreticulin antibody - ER Marker (ab2907)
    • Anti-Calreticulin antibody [EPR3924] - ER Marker (ab92516)

Specifications

Our Abpromise guarantee covers the use of ab91577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 0.056% Calcium chloride, 5% Glycerol (glycerin, glycerine), 0.877% Sodium chloride, 0.606% Tris

General Info

  • Alternative names

    • Autoantigen RO
    • CALR
    • CALR protein
    • CALR_HUMAN
    • Calregulin
    • Calreticulin
    • cC1qR
    • CRP55
    • CRT
    • CRTC
    • Endoplasmic reticulum resident protein 60
    • Epididymis secretory sperm binding protein Li 99n
    • ERp60
    • FLJ26680
    • grp60
    • HACBP
    • HEL S 99n
    • RO
    • Sicca syndrome antigen A
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin)
    • SSA
    see all
  • Function

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • Sequence similarities

    Belongs to the calreticulin family.
  • Domain

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • Cellular localization

    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Target information above from: UniProt accession P27797 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Calreticulin protein (ab91577)
    SDS-PAGE - Recombinant Human Calreticulin protein (ab91577)
    SDS-PAGE Analysis of ab91577 stained with Coomassie Blue.
    Lane 1: MW Marker
    Lane 2: 0.5 µg purified Calreticulin
    Lane 3: 1 µg purified Calreticulin
    Lane 4: 2 µg purified Calreticulin
    Lane 5: 5 µg purified Calreticulin
  • Western blot - Recombinant Human Calreticulin protein (ab91577)
    Western blot - Recombinant Human Calreticulin protein (ab91577)
    All lanes : Recombinant Human Calreticulin protein (ab91577) at 1/1000 dilution

    Lane 1 : MW Marker
    Lane 2 : 50ng Calreticulin
    Lane 3 : 100ng Calreticulin
  • Western blot - Recombinant Human Calreticulin protein (ab91577)
    Western blot - Recombinant Human Calreticulin protein (ab91577)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab91577? Please let us know so that we can cite the reference in this datasheet.

ab91577 has been referenced in 2 publications.

  • Chen B  et al. Calreticulin Binds to Fas Ligand and Inhibits Neuronal Cell Apoptosis Induced by Ischemia-Reperfusion Injury. Biomed Res Int 2015:895284 (2015). PubMed: 26583143
  • Hughes BG  et al. MMP-2 is localized to the mitochondria-associated membrane of the heart. Am J Physiol Heart Circ Physiol 306:H764-70 (2014). PubMed: 24375642

Customer reviews and Q&As

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Question


Inquiry: Regarding your product "Calreticulin protein (Human) (ab91577)", is this the full-length protein? Your website gives an amino acid start and end of '0'.

Read More

Abcam community

Verified customer

Asked on Aug 12 2013

Answer

Thank you for contacting us.


Yes, this is the full length protein. For full length proteins we have left the start and stop amino acids as 0, since there is not a start and stop like there is for a peptide.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Heather Allen

Abcam Scientific Support

Answered on Aug 12 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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