Recombinant human Cathepsin K protein (Active) (ab157067)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Cathepsin K protein (Active)
See all Cathepsin K proteins and peptides -
Biological activity
≥ 1.5 U/mg protein. Activity is lot specific.
One unit is defined as the amount of enzyme that will hydrolyze 1µmol Z-Phe-Arg-AMC substrate per min. at 37°C.
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Purity
> 95 % SDS-PAGE.
ab157067 was purified as full-length proenzyme, then auto-activated at low pH. -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MWGLKVLLLP VVSFALYPEE ILDTHWELWK KTHRKQYNNK VDEISRRLIW EKNLKYISIH NLEASLGVHT YELAMNHLGD MTSEEVVQKM TGLKVPLSHS RSNDTLYIPE WEGRAPDSVD YRKKGYVTPV KNQGQCGSCW AFSSVGALEG QLKKKTGKLL NLSPQNLVDC VSENDGCGGG YMTNAFQYVQ KNRGIDSEDA YPYVGQEESC MYNPTGKAAK CRGYREIPEG NEKALKRAVA RVGPVSVAID ASLTSFQFYS KGVYYDESCN SDNLNHAVLA VGYGIQKGNK HWIIKNSWGE NWGNKGYILM ARNKNNACGI ANLASFPKM -
Predicted molecular weight
26 kDa -
Amino acids
1 to 329
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Specifications
Our Abpromise guarantee covers the use of ab157067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.41% Sodium acetate, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.015% EDTA, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Cathepsin K
- Cathepsin O
- Cathepsin O1
see all -
Function
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Tissue specificity
Predominantly expressed in osteclasts (bones). -
Involvement in disease
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
Images
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Functional Studies - Recombinant human Cathepsin K protein (ab157067)
Each lane contains 1 ug of total protein during time-course activation at low pH.
Lane 1: Pre-Activation.
Lane 2: 0 min
Lane 3: 1 hour
Lane 4: 2 hours
Lane 5: 3 hours
Lane 6: 4 hours
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SDS-PAGE - Recombinant human Cathepsin K protein (ab157067)ab157067 on SDS-PAGE.
Lane 1: 1 ug.
Lane 2: 2 ug.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab157067 has been referenced in 3 publications.
- Yoon MC et al. Molecular Features of CA-074 pH-Dependent Inhibition of Cathepsin B. Biochemistry 61:228-238 (2022). PubMed: 35119840
- Ewanchuk BW et al. A non-immunological role for ?-interferon-inducible lysosomal thiol reductase (GILT) in osteoclastic bone resorption. Sci Adv 7:N/A (2021). PubMed: 33893096
- Yoon MC et al. Selective Neutral pH Inhibitor of Cathepsin B Designed Based on Cleavage Preferences at Cytosolic and Lysosomal pH Conditions. ACS Chem Biol 16:1628-1643 (2021). PubMed: 34416110