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Recombinant Human CBR3 protein (ab86848)

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SDS-PAGE - Recombinant Human CBR3 protein (ab86848)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Suitable for: WB, SDS-PAGE

    Description

    • Product name

      Recombinant Human CBR3 protein

    • Purity

      > 95 % SDS-PAGE.
      ab86848 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDP MPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENC SEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLG VTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAET PVYLALLPPD ATEPQGQLVH DKVVQNW

    Specifications

    Our Abpromise guarantee covers the use of ab86848 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • Carbonyl reductase (NADPH) 3
      • Carbonyl reductase (NADPH) 3 EC 1.1.1.184
      • Carbonyl reductase [NADPH] 3
      • Carbonyl reductase 3
      • CBR 3
      • CBR3
      • CBR3_HUMAN
      • EC 1.1.1.184
      • Epididymis secretory protein Li 25
      • hCBR3
      • HEL S 25
      • NADPH dependent carbonyl reductase 3
      • NADPH-dependent carbonyl reductase 3
      • SDR21C2
      • Short chain dehydrogenase/reductase family 21C member 2
      see all

    • Function

      Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).

    • Tissue specificity

      Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.

    • Sequence similarities

      Belongs to the short-chain dehydrogenases/reductases (SDR) family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CBR3 protein (ab86848)
      SDS-PAGE - Recombinant Human CBR3 protein (ab86848)
      15% SDS-PAGE showing ab86848 at approximately 33kDa (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab86848? Please let us know so that we can cite the reference in this datasheet.

    ab86848 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot Abreview for CBR3 protein (His tag)

     Excellent
    Abreviews
    abreview image
    Application
    Western blot

    Review text: We recently started using Abcam's CBR3 recombinant protein to confirm the activity of CBR3 primary antibodies. Additionally, we have successfully used this human protein to create standard curves (image included) for quantitative analysis of protein amounts in human liver tissue lysates.
    Sample: Human Recombinant protein (Recombinant protein)
    Loading amount: 0.03 µg
    Specification: Recombinant protein
    Gel Running Conditions: Non-reduced Denaturing (12% Gel)
    Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Other product details
    Dilution: 1/30
    Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-tween (0.05%)

    Primary antibody (in addition to 'CBR3 protein (His tag)')
    Name: Non-Abcam antibody was used: Carbonyl reductase 3 (C-16)
    Host species: Rabbit
    Clonality: Polyclonal
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/1000

    Secondary antibody
    Name: Non-Abcam antibody was used: Anti-Goat IgG (whole molecule)
    Host species: Rabbit
    Clonality: Polyclonal
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/10000

    Detection
    Detection method: ECL
    Exposure: 1 minute(s) and 0 second(s)
    Bands: Specific: 30 kDa
    Positive control: No positive control was necessary given that the recombinant CBR3 protein comprised our protein of interest.

    Additional data
    Additional Notes: Nitrocellulose membranes were used in our WB. The image of the standard curve shows the CBR3 recombinant protein in increasing amounts from left to right (0.03, 0.07, and 0.10 ug) and a protein ladder on the far right as detected using the conditions outlined above.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 10 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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