Recombinant human CD19 protein (Active) (Biotin) (ab167717)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: WB, ELISA, SDS-PAGE
Description
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Product name
Recombinant human CD19 protein (Active) (Biotin)
See all CD19 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA.
Immobilized FMC63 mAb at 2 μg/mL (100 μL/well) binds Biotinylated Human CD19, Fc tag, with a linear range of 0.15-2.5 μg/mL. -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LFLTPMEVRP EEPLVVKVEE GDNAVLQCLK GTSDGPTQQL TWSRESPLKP FLKLSLGLPG LGIHMRPLAI WLFIFNVSQQ MGGFYLCQPG PPSEKAWQPG WTVNVEGSGE LFRWNVSDLG GLGCGLKNRS SEGPSSPSGK LMSPKLYVWA KDRPEIWEGE PPCLPPRDSL NQSLSQDLTM APGSTLWLSC GVPPDSVSRG PLSWTHVHPK GPKSLLSLEL KDDRPARDMW VMETGLLLPR ATAQDAGKYY CHRGNLTMSF HLEITARPVL WHWLLRTGGW K -
Predicted molecular weight
56 kDa including tags -
Amino acids
20 to 291 -
Tags
Fc tag C-Terminus -
Additional sequence information
A Chimeric Protein: Biotinylated CD19-Fc is fused with Fc fragment of human IgG1 at the C-terminus.
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Conjugation
Biotin
Specifications
Our Abpromise guarantee covers the use of ab167717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
SDS-PAGE
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Form
Lyophilized -
Additional notes
The biotin to protein ratio is 2.5-3.5 as determined by the HABA assay.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 100 µg/ml.
General Info
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Alternative names
- Antibody deficiency due to defect in CD19
- Antibody deficiency due to defect in CD19, included
- AW495831
see all -
Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human CD19 protein (Active) (Biotin) (ab167717)
Immobilized Anti-Mouse FMC63 Mab at 2 μg/mL (100 μL/well) binds ab167717.
Linear range: 0.039-0.625 μg/mL (QC tested).
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Functional Studies - Recombinant human CD19 protein (Active) (Biotin) (ab167717)Immobilized Anti-Mouse FMC63 Mab at 2 μg/mL (100 μL/well) binds ab 167717.
Linear range: 0.039-0.625 μg/mL (QC tested).
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SDS-PAGE - Recombinant human CD19 protein (Active) (Biotin) (ab167717)Reduced ab167717 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >95%.
The protein migrates as 56-66 kDa under reducing conditions, due to glycosylation.
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Flow Cytometry - Recombinant human CD19 protein (Active) (Biotin) (ab167717)HEK293 cells were transfected with FCM63-scFv and RFP tag . 2x105 of the cells were first incubated with:
A. Biotinylated protein control.
B. 10 μg/ml of ab167717.
C. 10 μg/ml of ab167717 and FMC63(Mouse anti-CD19 antibody). FITC Streptavidin was used to analyse with FACS. RFP was used to evaluate CAR(FMC63-scFv) expression and FITC was used to evaluate the binding activity of ab167717.
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Flow Cytometry - Recombinant human CD19 protein (Active) (Biotin) (ab167717)Human T cells were lentivirally transduced with anti-CD19 CAR and cultured for 11 days.
Eleven days post-transduction, 1e6 cells were stained for the expression of CD3 and anti-CD19 CAR with FITC anti-human CD3 antibody and ab167717, followed by PE-conjugated streptavidin, respectively.
A. Non-transduced T cells were used as a control for gating of CAR expression. (Data are kindly provided by Beijing Bowei Huaen Medical Technology Co. Ltd.)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab167717 has not yet been referenced specifically in any publications.
