Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Recombinant Human CD3 epsilon protein (ab114153)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human CD3 epsilon protein (ab114153)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: ELISA, WB, SDS-PAGE

    Description

    • Product name

      Recombinant Human CD3 epsilon protein
      See all CD3 epsilon proteins and peptides

    • Expression system

      Wheat germ

    • Accession

      P07766

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        DGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQRNDKNIGGDEDD KNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENC MEMDVMSVATIVIVDICITGGLLLLVYYWSKNRKAKAKPVTRGAGAGGRQ RGQNKERPPPVPNPDYEPIRKGQRDLYSGLNQRRI

      • Predicted molecular weight

        46 kDa including tags

      • Amino acids

        23 to 207

      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab114153 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Western blot

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • CD3 epsilon
      • CD3e
      • CD3e antigen
      • CD3E antigen epsilon polypeptide
      • CD3e antigen epsilon polypeptide (TiT3 complex)
      • CD3E antigen, epsilon subunit
      • CD3e molecule epsilon
      • CD3e molecule, epsilon (CD3 TCR complex)
      • CD3e molecule, epsilon (CD3-TCR complex)
      • CD3E_HUMAN
      • IMD18
      • T cell antigen receptor complex epsilon subunit of T3
      • T cell surface antigen T3/Leu 4 epsilon chain
      • T cell surface glycoprotein CD3 epsilon chain
      • T-cell surface antigen T3/Leu-4 epsilon chain
      • T-cell surface glycoprotein CD3 epsilon chain
      • T3E
      • TCRE
      see all

    • Function

      The CD3 complex mediates signal transduction.

    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CD3 epsilon protein (ab114153)
      SDS-PAGE - Recombinant Human CD3 epsilon protein (ab114153)
      12.5% SDS-PAGE analysis of ab114153, stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab114153? Please let us know so that we can cite the reference in this datasheet.

    ab114153 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    How can I measure the binding affinity of this antibody?

    Read More
    Answer

    Thank you for contacting us.

    The following paper may be helpful in determining the binding affinity of this antibody. Basically, this information can be measured in an indirectELISA assay using the antibody and a purified antigen such as ab114153. I hope this helps, please let me know if you need any additional information or assistance.

    http://www.enzim.hu/data/pdf/12379321.pdf

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com