Recombinant Human CD34 protein (ab182830)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human CD34 protein
See all CD34 proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab182830 was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MASMTGGQQMGRGHHHHHHGNLYFQGGEFSLDNNGTATPELPTQGTFSNV STNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSV YGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTS LATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEF KKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTE ISSKLQLMKKHQSDLKKLGILDFTEQDVASHQSYSQKT -
Predicted molecular weight
31 kDa including tags -
Amino acids
32 to 290 -
Tags
His-T7 tag N-Terminus -
Additional sequence information
Constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. NP_001764
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab182830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and Glycerol.
General Info
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Alternative names
- CD34
- CD34 antigen
- CD34 molecule
see all -
Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab182830 has not yet been referenced specifically in any publications.