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Recombinant human CDA protein (ab99441)

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SDS-PAGE - Recombinant Human CDA protein (ab99441)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant human CDA protein

    • Biological activity

      Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of required to deaminate 1.0 pmole of cytidine per min at pH 7.5 at 25C.

    • Purity

      > 90 % SDS-PAGE.
      ab99441 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P32320

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMAQKRPACTLKPECVQQLLVCSQEAKQSAY CPYSHFPVGAALLTQEGRIFKGCNIENACYPLGICAERTAIQKAVSEGYK DFRAIAIASDMQDDFISPCGACRQVMREFGTNWPVYMTKPDGTYIVMTVQ ELLPSSFGPEDLQKTQ

      • Predicted molecular weight

        18 kDa including tags

      • Amino acids

        1 to 146

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab99441 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0584% EDTA, 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • CDD
      • Cytidine aminohydrolase
      • Cytidine deaminase
      • EC 3.5.4.5

    • Relevance

      CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes.

    Images

    • SDS-PAGE - Recombinant Human CDA protein (ab99441)
      SDS-PAGE - Recombinant Human CDA protein (ab99441)
      15% SDS-PAGE showing ab99441 at approximately 18.3kDa (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab99441? Please let us know so that we can cite the reference in this datasheet.

    ab99441 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Customer phoned to ask if this product is in an active form.

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    Answer

    Thank you for contacting Abcam regarding the ab99441.

    I have had the opportunity to speak with the lab regarding your question. I have been told thatit may be active, but regrettably, it hasn’t been tested yet. Although it is scheduled to be tested eventually, we can’t give you a specific date on when we will have the results.

    I hope this information is helpful. Please contact me if you have any questions.

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